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Application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing periodontitis

A technology of green olive and extract, which is applied in the field of plant extracts for the prevention and treatment of periodontitis, can solve the problem that no green olive extract inhibits oral anaerobic bacteria, and achieves the reduction of biofilm formation, convenient prevention and significant prevention The effect of action

Inactive Publication Date: 2013-06-05
LOGISTICS UNIV OF CAPF
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

But so far there is no report on the inhibition of oral anaerobic bacteria by green olive extract

Method used

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  • Application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing periodontitis
  • Application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing periodontitis
  • Application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing periodontitis

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preparation example Construction

[0023] The preparation steps of the green olive extract are as follows: taking green olive branches, leaves or fruits as raw materials, carrying out reflux extraction with water or ethanol aqueous solution, filtering, concentrating the filtrate under reduced pressure to obtain a concentrate, and drying to obtain the green olive extract.

[0024] The preparation parameters of the green olive extracts of Examples 1-14 are shown in Table 1.

[0025] Table 1

[0026]

[0027]

Embodiment 15

[0028] Example 15 Test of green olive extract in vitro inhibition of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum induced by periodontitis

[0029] Porphyromonas gingivalis (Porphyromonas gingivalis) ATCC33277 was purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0030] Intermediate Prevotella (Prevotella intermedia) ATCC25611 was purchased from Beijing Beinan Chuanglian Biotechnology Research Institute.

[0031] and Fusobacterium nucleatum (Fusobacterium nucleatum) ATCC10953 were purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0032] Test method: Porphyromonas gingivalis ATCC33277, Prevotella intermedia ATCC25611 and Fusobacterium nucleatum ATCC10953 were taken out from the -80°C refrigerator respectively, revived routinely, and inoculated in BHI Agar medium, cultured anaerobically at 37°C (80% N 2 , 10%H 2 , 10% CO 2 ) 48h, pick a single colony and replant it in BHI liquid medium, and cu...

Embodiment 16

[0037] Example 16 Effects of different concentrations of green olive extracts on Porphyromonas gingivalis biofilm formation

[0038] Test method: In this experiment, the plant extract prepared in Example 10 was used to carry out the following tests. The blank control group was Porphyromonas gingivalis ATCC33277 bacterial suspension (200 μL per well). The negative control group was a polyethylene 96-well plate containing absolute ethanol (10 μL per well) containing Porphyromonas gingivalis ATCC33277 suspension (190 μL per well). In the experimental group, 1 / 4MIC, 1 / 2MIC, MIC, 2MIC green olive extracts (10 μL per well) were added to polyethylene 96-well plates containing Porphyromonas gingivalis ATCC33277 suspension (190 μL per well). In triplicate, the same concentration level was repeated 4 times. 37 ℃ anaerobic culture for 48h. Suck out the bacterial suspension in the wells with a micropipette, gently wash the wells 3 times with PBS to remove planktonic bacteria, add fresh...

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Abstract

The invention discloses application of green olive extract in preparation of drugs or food or daily necessities for preventing and curing periodontitis. Experiments prove that green olive extract can obviously suppress porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum which cause periodontitis, the porphyromonas gingivalis, the prevotella intermedia and the fusobacterium nucleatum are prevented from forming biological membrane, so that the green olive extract has an obvious preventing and curing function on periodontitis. The green olive extract can be prepared into various medicines or food or daily necessities, and thus the periodontitis is prevented and cured conveniently..

Description

technical field [0001] The invention relates to a plant extract for preventing and treating periodontitis, in particular to the application of green olive plant extract in the fields of food, medicine and oral preparations for preventing and treating periodontitis. Background technique [0002] The plant extract involved in the present invention is from the plant extract of green olive which is abundant in my country. The green olive tree Canarium album (Lour.) Raeusch. is a kind of economic fruit tree planted in parts of southern my country. Its mature and dry fruit is oval and blue in color. It is also known as green fruit, green seed, white olive, green olive, etc. The effective active ingredients are olive polyphenols, which have the functions of clearing heat, relieving throat, promoting body fluid, detoxification, immune regulation, lowering blood pressure, lowering blood fat, and scavenging free radicals. It is used to treat sore throat, cough, polydipsia, fish and cra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/32A61K8/97A61P1/02A61Q11/00A23L1/29A23G3/48A23G4/06A61K127/00A61K131/00A61K135/00A23L33/00
Inventor 刘岱琳赵艳敏陈虹延慧君马卫俊高颖
Owner LOGISTICS UNIV OF CAPF
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