Preparation method of nano fiber double layer support used for skin tissue engineering

Abstract

The invention relates to a preparation method of a nano fiber double layer support used for skin tissue engineering, which belongs to the biology medical material technical field. Polylactic acid, chitosan and polycaprolactone are taken as the support materials, a thermotropic phase separating technology and a high pressure static spinning technology are combined to prepare the porous double layer network support having a nano fiber structure. The bottom layer of the double layer support is an polylactic acid nano fiber support prepared by the thermotropic phase separating technology, fiber diameter is between 200-750nm, the surface layer is the chitosan-polycaprolactone composite nano fiber film prepared by the static spinning technology, and the fiber diameter is about 200nm. The support stimulates a cuticle and corium double layer structure of human body skin from a microstructure, and is suitable for restoration of the human body skin.

Description

technical field [0001] The invention relates to a method for preparing a nanofiber double-layer scaffold for skin tissue engineering, and belongs to the technical field of biomedical materials. Background technique [0002] The main purpose of the research and preparation of biomedical materials is to use various natural or synthetic materials to make artificial organs or medical devices to replace and repair damaged tissues and organs of the human body, and finally realize the normal physiological functions of tissues and organs. [0003] As the largest organ of the human body, the skin plays a role in protecting the human body from the invasion of external objects. Damage to the skin can lead to the invasion of bacteria and pathogens, and severe cases can lead to death. Therefore, in the past three decades, a large number of studies on skin scaffolds have been carried out in the fields of biomedical materials and tissue engineering. So far, a variety of commercial produc...

AI Technical Summary

Problems solved by technology

However, most of the double-layer scaffolds used in the current literature and published patents are microporous structures, and are prepared by a single method.

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