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Bacillus amyloliquefaciens and application of bacillus amyloliquefaciens in peanut rot disease preventive treatment

A technology of amylolytic spores and bacillus, applied in the direction of application, microbe-based methods, chemicals for biological control, etc., can solve the problems of harming the interests of peanut growers and poor long-term control effect of peanut fruit rot

Inactive Publication Date: 2013-06-05
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From 2006 to 2011, the disease was discovered in my country's Hebei, Shandong, Jilin, Henan and other peanut planting areas, with a rotten fruit rate of 30-100%, which seriously damaged the interests of local peanut growers
However, the bacillus preparations currently on the market have poor long-term control effects on peanut fruit rot

Method used

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  • Bacillus amyloliquefaciens and application of bacillus amyloliquefaciens in peanut rot disease preventive treatment
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  • Bacillus amyloliquefaciens and application of bacillus amyloliquefaciens in peanut rot disease preventive treatment

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1 Isolation and Identification of Bacillus amyloliquefaciens

[0044] In July 2010, Bacillus amyloliquefaciens was isolated from the shells of peanuts collected in the peanut planting area of ​​Daming County, Hebei Province through confrontation culture with the pathogenic bacteria of peanut fruit rot.

[0045] Specific method: Fresh and healthy peanut pods collected in the peanut planting area of ​​Daming County, Hebei Province were washed, sterilized with 0.1% mercuric chloride for 30 s, surface sterilized with 75% ethanol for 30 s, rinsed with sterilized water for 4 times, and 1 g of the shell was cut and sterilized. Crush in a bacteria mortar, add 10mL of sterilized water, take 100μL and evenly spread it on the PDA medium containing the meridian of the pathogenic bacteria of peanut fruit rot, incubate at 30°C for 48-72 hours, pick the colony that produces the inhibition zone and carry out Purification culture.

[0046]

[0047] Physiological and biochemi...

Embodiment 2

[0051] The fermentation process of embodiment 2 bacterial manure

[0052] Pick a single colony of Bacillus amyloliquefaciens on the PDA plate and place it in 3 mL of potato dextrose liquid medium, and culture it on a constant temperature shaker at 30°C and 220 rpm for 16 hours to obtain the first culture;

[0053] Transfer the first culture to potato dextrose liquid culture medium with 1% inoculum amount, and place it in a constant temperature shaker at 30°C and 220rpm for 26 hours to obtain the second culture;

[0054] The second culture is used as the seed liquid for solid-state shake flask fermentation, and transferred to the solid-state shake flask fermentation medium at 12%, and cultured at 30°C for 120 hours after shaking; the solid-state shake flask fermentation medium for the bacterial fertilizer is: wheat bran 26 g; cottonseed husk 9 g; peanut cake powder 4.0 g; water 45 mL.

[0055] Dry in the shade and pack into bags.

Embodiment 3

[0056] The fermentation process of embodiment 3 bacterial manure

[0057] Pick a single colony of Bacillus amyloliquefaciens on the PDA plate and place it in 3 mL of potato dextrose liquid medium, and culture it on a constant temperature shaker at 37°C and 200 rpm for 12 hours to obtain the first culture;

[0058] Transfer the first culture to potato dextrose liquid medium according to 10% inoculum amount, and culture it under constant temperature shaker at 37°C and 200rpm for 22 hours to obtain the second culture;

[0059] The second culture is used as the seed liquid for solid-state shake flask fermentation, and transferred to the solid-state shake flask fermentation medium at 8%, and cultured at 37°C for 140 hours after shaking; the solid-state shake flask fermentation medium for the bacterial fertilizer is: wheat Bran 30 g; cottonseed husk 7 g; peanut cake powder 2.5 g; water 44.5 mL.

[0060] Dry in the shade and pack into bags.

[0061]

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Abstract

The invention relates to the microbial preventive treatment field, in particular to a bacillus amyloliquefaciens bacterial strain, solid state fermentation bacteria manure, and application of bacillus amyloliquefaciens in peanut rot disease preventive treatment. Bacterial manure which contains bacillus amyloliquefaciens with CGMCC No.5436 preservation number is diluted 100-1000 times and hole applied to peanuts, a field colonization growth promoting experiment proves that the bacillus amyloliquefaciens can be colonized inside peanut rhizosphere soil and roots and have distinct growth-promoting effect on the roots, and preventive treatment effect on root diseases achieves more than 60%. A field disease prevention experiment proves that preventive treatment effect on the peanut rot disease achieves more than 50%, emergence rate is increased by 10-20%, yield is increased by 6-12%, and disease control is achieved, and meanwhile the purposes of peanut retention and yield increase are achieved. The bacillus amyloliquefaciens can be colonized inside the peanut rhizosphere soil and the roots, good preventive treatment effect on the peanut rot disease is achieved, and then the bacillus amyloliquefaciens plays a role in growth promotion and yield increase, and meanwhile spores of the bacillus amyloliquefaciens is high in content, little in use level, non-toxic, free of pathogenicity, safe to people and livestock, free of environmental pollution, and low in production cost.

Description

technical field [0001] The invention relates to the field of microbial prevention and control, in particular to bacillus amyloliquefaciens strains and their application in the prevention and treatment of peanut fruit rot. Background technique [0002] Peanut fruit rot is a soil-borne disease that occurs in the middle and late stages of peanut growth. Neocosmospora sp.) A disease that infects peanuts and causes peanuts to rot. The characteristics of the disease are as follows: (1) The diseased pods are mainly mid-late stage pods and full fruits; (2) When the initial occurrence, peanut pods The mesocarp and exocarp of the pod become dark brown spots, and the endocarp and seeds are normal; (3) When the disease is severe, the entire pericarp and seeds of the pod turn black and rot; (4) The root outer cortex turns black, while the aerial part remains unchanged Or greener. From 2006 to 2011, the disease was discovered successively in my country's peanut planting areas such as He...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C05F11/08C12R1/07
Inventor 郭巍孙伟明冯丽娜陆秀君李瑞军徐大庆赵丹于浩海刘大群
Owner HEBEI AGRICULTURAL UNIV.
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