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Bacillus subtilis bacterial strains and application thereof in microbial power generation

A technology of Bacillus subtilis and microorganisms, applied in the field of microorganisms, to achieve the effects of sewage purification treatment, improvement of anaerobic experimental conditions, and expansion of the scope

Inactive Publication Date: 2013-06-05
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No reports of facultative anaerobic bacteria having electrogenic activity in the prior art

Method used

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  • Bacillus subtilis bacterial strains and application thereof in microbial power generation
  • Bacillus subtilis bacterial strains and application thereof in microbial power generation
  • Bacillus subtilis bacterial strains and application thereof in microbial power generation

Examples

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Embodiment 1

[0029] Isolation of strain MMR-1

[0030] The microbial fuel cell adopts a double-chamber H-shaped bottle system, passing two 250ml inorganic glass bottles with a lid with a diameter of 1.6cm 2 The glass tubes are connected, and two inorganic glass bottles with lids are used as the anode and cathode chambers, carbon paper is used as the electrode, and cyanobacteria are used as the anode catalyst to inoculate the anode chamber. The carbon paper is fixed in the cathode and anode chambers by graphite clamps. The distance between the two electrodes is 12cm 2 , Connect with external resistance or multimeter through copper wire.

[0031] After the microbial fuel cell reacts, a biofilm is enriched on the anode carbon paper. Place the anode carbon paper under the scanning electron microscope for observation, such as figure 1 As shown, the biofilm is mainly composed of bacteria. The biofilm on the anode carbon paper was inoculated with multiple streaks to obtain purified strains, and the ...

Embodiment 2

[0040] Embodiment 2 of the present invention provides a microbial fuel cell, which includes an anode chamber, a cathode chamber, and an external circuit. The anode chamber contains an anolyte, an anode, and electricity-generating microorganisms. The electricity-generating microorganisms are MMR- obtained in Example 1. 1 strain or biologically pure culture with all the identifying characteristics of MMR-1 strain.

[0041] Further, the anolyte is a mixture of 195 ml of BG11 medium and sodium acetate, the anode is carbon paper, the cathode is an air electrode catalyzed by Pt / C, and the cathode compartment contains a phosphate buffer solution with a concentration of 50 mM.

[0042] The anolyte in this embodiment can also be broth medium or sewage, especially when sewage is used as anolyte, in addition to power generation, it can also decompose organic matter in the sewage, which not only realizes the conversion of chemical energy into electric energy, but also realizes sewage purificati...

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Abstract

The invention relates to the field of microbial technology, in particular to bacillus subtilis bacterial strains. The bacterial strain is MMR-1, and the preservation number of the bacterial strain is CGMCC No.6871. The invention further provides a microbial fuel cell which comprises an anode chamber, a cathode chamber, and an external circuit, wherein the anode chamber comprises anode solutions, an anode, and electricity generating microbes, and the electricity generating microbes are biological pure cultures of the bacillus subtilis bacterial strains or biological pure cultures which have all the identifying characteristics of the bacillus subtilis bacterial strains. The bacillus subtilis bacterial strains are facultative anaerobes and have a function of generating electricity, so that the rang of electricity generating microbes is expanded, and anaerobic experiment conditions can be improved through the preparation of the microbial fuel cell by using the bacillus subtilis bacterial strains.

Description

【Technical Field】 [0001] The present invention relates to the technical field of microorganisms, in particular to a new Bacillus subtilis strain and its application in microbial fuel cells and microbial power generation. 【Background technique】 [0002] With the increasing severity of global warming and fossil fuel exhaustion, the research on alternative and renewable energy sources has received more and more attention. In recent years, a microbial fuel cell (MFC), a power generation device that can directly convert chemical energy into electrical energy, has gradually become a research hotspot in the emerging energy field with its unique advantages. MFC combines microbial technology with battery technology, and uses microorganisms as a catalyst to directly convert the chemical energy of organic matter into electricity. It has the dual functions of power generation and waste disposal. In addition to high-concentration organic wastewater, MFC can also use domestic sewage and other...

Claims

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Application Information

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IPC IPC(8): C12N1/20H01M8/16C12R1/125
CPCY02E60/527Y02E60/50Y02P70/50
Inventor 邓宗武马美荣曹利敏毛健
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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