Micro-fluidic chip culture device of cell capable of being stretched mechanically and application thereof

Abstract

The invention discloses a micro-fluidic chip culture device of cells capable of being stretched mechanically. The cell culture device comprises a cell culture layer, a polydimethylsiloxane (PDMS) film and a liquid flow control layer, wherein the cell culture layer, the PDMS film and the liquid flow control layer are overlaid in sequence. At least one micro channel is arranged on the liquid flow control layer, at least one chamber is arranged on the micro channel, and the height of the at least one chamber is equal to that of the at least one micro channel. Cell culture ponds are arranged on the cell culture layer, the number of the cell culture ponds is equal to that of the at least one chamber, the PDMS film is arranged at the bottom of the cell culture pond, and the shape of the cell culture pond is identical to that of the at least one chamber. Along the direction from the liquid flow control layer to the cell culture layer, the positions of the cell culture ponds and the at least one chamber correspond mutually, the inlet and the outlet of the at least one micro channel penetrate through the PDMS film and the cell culture layer in sequence to be communicated with the outside. The micro-fluidic cell culture device can be used for researching the response of the cells to mechanical stretching force, and is simple, sensitive and reliable.

Description

technical field [0001] The invention relates to a mechanically stretchable microfluidic chip cell culture device and an application thereof, belonging to the technical field of cell culture in a lab-on-a-chip system. Background technique [0002] Cell culture is an important technology in the fields of biology and health related research. With the rapid development of life sciences, cell culture has become an important basis for the research of cell biology, molecular biology, genetics and immunology. In order to deeply explore the growth and activity of cells, the pathology of related diseases and the pharmacological (toxicological) mechanism of drugs, it is of great significance to develop different targeted cell culture methods. Currently, various culture flasks (dishes) are widely used for cell culture, which is limited to a single static culture environment. However, under normal physiological conditions, cells are often in a complex mechanical environment, such as bo...

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Problems solved by technology

In addition, there is also a loading device that presses down the elastic polyacrylamide gel through a circular hollow pressure head, so that it produces uniform and stable equibiaxial deformation to stretch the cells, and investigates the liquefaction and solidification of smooth muscle cells by tensile strain (Chen, C.; Krishnan, R.; Zhou, E.; Ramachandran, A.; Tambe, D.; Rajendran, K.; Adam, R.M.; Deng, L.; Fredberg, J.J. PloS one 2010, 5, e12035.), but the above devices that generate mechanical tensile stress on cells are complex, difficult to operate, and difficult to promote

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