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Electrochemical biosensor or detection of dopamine and its preparation method

A biosensor, electrochemical technology, applied in the field of electrochemical biosensors, can solve the problems of unfavorable transportation and storage of biosensors, increase the interference of interfering molecular detection, change sensor sensitivity, etc., achieve low detection limit, improve catalytic performance. Ability, effect of simple steps

Inactive Publication Date: 2013-06-12
HIGH & NEW TECH RES CENT OF HENAN ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The most important point is that most of the protective film materials modified by electrochemical biosensors are easily inactivated or even peeled off from the electrode surface, which will easily lead to changes in sensor sensitivity, decrease in linear range, and even increase the interference of interfering molecules. detection interference
As a result, not only is it not conducive to the transportation and storage of biosensors, but it also increases the cost per test

Method used

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  • Electrochemical biosensor or detection of dopamine and its preparation method
  • Electrochemical biosensor or detection of dopamine and its preparation method
  • Electrochemical biosensor or detection of dopamine and its preparation method

Examples

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Embodiment 1

[0025] An electrochemical biosensor for detecting dopamine. The biosensor is obtained by electropolymerizing PEDOT on a platinum disk electrode by an electrochemical method, and then combining it with nano gold through electrostatic interaction. The preparation method of the electrochemical biosensor for detecting dopamine, it comprises the steps:

[0026] 1) Immobilization of PEDOT thin film on the surface of platinum disk electrode: with hydrophilic ionic liquid BMIMBF 4As the electrolyte and supporting electrolyte, the concentration of monomer EDOT is kept at 0.1 mol / L, and the solution is kept in a nitrogen atmosphere throughout the reaction. In the three-electrode system, using cyclic voltammetry, in the stirring state, within the working potential range of -0.6 ~ 1.6 V with silver wire as a reference, scan 10 times circularly, and polymerize to obtain a blue PEDOT film; Step 1 The platinum disk working electrode described in ) has been pre-treated as follows: the platin...

Embodiment 2

[0035] An electrochemical biosensor for detecting dopamine. The biosensor is obtained by electropolymerizing PEDOT on a platinum disk electrode by an electrochemical method, and then combining it with nano gold through electrostatic interaction. The preparation method of the electrochemical biosensor for detecting dopamine, it comprises the steps:

[0036] 1) Immobilization of PEDOT thin film on the surface of platinum disk electrode: with hydrophilic ionic liquid BMIMBF 4 As the electrolyte and supporting electrolyte, the concentration of monomer EDOT is kept at 0.3 mol / L, and the solution is kept in a nitrogen atmosphere throughout the reaction. In the three-electrode system, using cyclic voltammetry, in the stirring state, within the working potential range of -0.6 ~ 1.6 V with silver wire as a reference, scan 8 times circularly, and polymerize to obtain a blue PEDOT film; Step 1 The platinum disk working electrode described in ) has been pre-treated as follows: the platin...

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Abstract

The invention discloses an electrochemical biosensor for detection of dopamine and its preparation method. The preparation method of the biosensor includes: first employing an electrochemical technique to conduct electropolymerization on a platinum disk electrode surface so as to make the surface coated with a layer of poly(3, 4-ethylenedioxythiophene) film, and then combining the film with nanogold through electrostatic interaction. The biosensor has a superstrong stable life, its signal can be maintain invariant for 8 months, thus far surpassing the 3-month life of current traditional dopamine electrochemical sensors. In addition, the biosensor has a very wide detection range of 6.0*10<-6>-0.13mol / L, a detection limit of 1.5*10<-6>mol / L, and a response time of only 2.5s.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors, and specifically relates to an ultra-stable electrochemical biosensor for detecting dopamine based on poly(3,4-ethylenedioxythiophene) / nano-gold conductive composite film and a preparation method thereof . Background technique [0002] Dopamine is responsible for the information transmission of the central nervous system of the brain in the human central nervous system. Dopamine is closely related to several neurological disorders, such as schizophrenia, Huntington's disease and Parkinson's symptoms. Therefore, it is of great significance to accurately detect the physiological content of dopamine in the human body to design the diagnosis and treatment schemes of the above-mentioned diseases and to judge the efficacy of the treatment schemes. At present, studies have reported methods for the determination of dopamine, such as liquid chromatography-fluorescence spectroscopy, ga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 刘珂珂刘清褚艳红刘冲
Owner HIGH & NEW TECH RES CENT OF HENAN ACAD OF SCI
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