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Method for detecting tervalence gold ion

A technology of gold ion and fluorescent red, which is applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of expensive, tedious process, and low selectivity of gold ion, and achieve low cost, simple detection method, high sensitivity and selectivity sexual effect

Inactive Publication Date: 2013-06-19
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But they have some disadvantages: the probe needs to be synthesized by tedious and expensive organic synthesis, the selectivity to gold ions is not high, and the detection is carried out in organic phase or semi-aqueous phase, etc.

Method used

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  • Method for detecting tervalence gold ion
  • Method for detecting tervalence gold ion
  • Method for detecting tervalence gold ion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Prepare HEPES buffer solution with pH=7.0 and concentration of 10mM, and prepare 2mM FRGK solution with ethanol; add 2mL of HEPES buffer solution and 2μL of FRGK ethanol solution to a clean fluorescence cuvette, and take Au 3+ The solution is gradually added to the cuvette with a micro-injector, and it is detected on the fluorescence spectrophotometer while adding the sample. 3+ The addition of 480, 583nm fluorescence intensity gradually weakened. Fluorescence emission map see figure 1 .

Embodiment 2

[0020] Prepare HEPES buffer solution with pH=7.0 and concentration of 10mM, and prepare 2mM FRGK solution with ethanol; add 2mL of HEPES buffer solution and 2μL of FRGK ethanol solution to each of the 25 fluorescence cuvettes, and then add 50 molar equivalents of Au 3+ , and 500 molar equivalents of various other cations (Cu + , Ca 2+ ,Fe 2+ ,Zn 2+ , Ni 2+ , Bi 3+ ,Co 2+ ,VO 2+ ,Mn 2+ , Ru 3+ ,Cd 2+ ,Pb 2+ , Ag + , La 3+ , Ce 4+ ,Yb 3+ ,Cr 2+ ,Er 3+ ,Sn 2+ ,Nd 3+ ,Zr 4+ ,Pd 2+ ,Fe 3+ , and Eu 3+ ), detected on a fluorescence spectrophotometer, draw the histogram of the 583nm fluorescence intensity corresponding to different cations, and obtain the fluorescence emission diagram, see figure 2 (Au 3+ It makes the fluorescence intensity of FRGK change from 583 to about 50, and other ions basically do not cause the change of the fluorescence intensity of FRGK).

[0021] It has been proved by experiments that other cations do not interfere with the Au 3+ d...

Embodiment 3

[0023] Prepare 2 mM Au with distilled water 3+ solution, add 2 mL of HEPES buffer solution and 2 μL of FRGK ethanol solution to the fluorescence cuvette, and gradually add Au 3+ The volume of the solution is 2.5, 5, 10, 20, 40, 50, 60, 80, 90uL, and the corresponding fluorescence intensity F at 583nm measured on the fluorescence spectrometer is 546, 533, 505, 463, 336, 291, 241, 136, 61, with Au 3+ Concentration is the abscissa, relative fluorescence intensity F 0 -F(F 0 ﹦581) draw the graph for the ordinate, and get Au 3+ The working curve of the concentration; the linear regression equation is: F 0 -F=22.59+5.21c (the unit of c is 10 -6 mol / L);

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Abstract

The invention provides a method for detecting a tervalence gold ion, particularly, a method based on fluorescein red GK (FRGK) which can be obtained in business for detecting the tervalence gold ion. The specific detection method includes: taking the FRGK as fluorescent reagent, and quantificationally detecting content of the tervalence gold ion in 2-hydroxyethyl (HEPES) solution with a potential of hydrogen (PH) value of 7.0. The method shows high sensitivity and selectivity for the tervalence gold ion. Detection process is simple, easy and rapid, and detection result is accurate.

Description

Technical field: [0001] The present invention relates to Au 3+ The detection and analysis technology specifically belongs to a quantitative detection of Au based on fluorescent red fluorescent red GK (FRGK). 3+ Methods. Background technique: [0002] The study of gold-related chemistry is currently the most rapidly developing field because of its relevance to many aspects of materials science, including catalysis, selective oxidation, nanodiagnostics and detection, and photophysical properties of noble metals. Gold is also used in anti-rheumatic medicines. Gold can inhibit macrophages and leukocytes and anti-rheumatic joint inflammation. At the same time, since gold chloride salt can cause damage to the liver, kidney and peripheral nervous system, gold has potential toxicity to the human body. Gold salt concentrations greater than 200 μM are highly toxic. In 1 mL of saline, when HAuCl 4 At a concentration of 10-15μM, it can cause agglutination and hemolysis of red bloo...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 阴彩霞杨瑜涛霍方俊
Owner SHANXI UNIV
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