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Rapid detective reagent strip of treponema pallidum immunoglobulin m (IgM) antibody and preparation method thereof

A technology for treponema pallidum and detection reagents, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of simple and fast operation, high sensitivity and strong specificity

Active Publication Date: 2013-06-19
天津中新科炬生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a negative TP-IgM does not completely rule out infectivity, a positive TP-IgM must suggest that the patient is infectious

Method used

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  • Rapid detective reagent strip of treponema pallidum immunoglobulin m (IgM) antibody and preparation method thereof
  • Rapid detective reagent strip of treponema pallidum immunoglobulin m (IgM) antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] see figure 1 , a rapid detection reagent strip for Treponema pallidum IgM antibody comprising a sample pad (1), a colloidal gold pad (2), an NC film (3), a sample suction pad (4) and a plastic plate (7), and the colloidal gold pad is marked with T1 antibody and TP fusion protein, the anti-human IgM μ chain monoclonal antibody coated on the NC membrane constitutes the T line (5) and the goat anti-mouse IgG antibody constitutes the C line (6), the sample pad, colloidal gold pad, NC membrane Paste the sample pad on the plastic plate to form a detection reagent strip.

[0028] The detection reagent strip is prepared by the following method:

[0029] 1. Preparation of Colloidal Gold Pads

[0030] Prepare the colloidal gold solution that diameter is 30-50nm with chloroauric acid-trisodium citrate reduction method, 0.2MK CO Regulate the pH of colloidal gold solution to be 8.5-9.5, then be placed on the magnetic stirrer and stir slowly, press every 100ml solution containing ...

Embodiment 2

[0038] Similar to Example 1, the difference is that the labeling and coating proteins are reversed, that is, the colloidal gold pad is labeled with an anti-human IgM μ chain monoclonal antibody, and the detection area on the NC membrane is coated with TP recombinant antigen as a T line (5), and at the same time, it is coated with the labeled plus The sample loading indicator line (8) at the sample position, for details, refer to figure 2 . When testing, first add 3 to 5ul of the standard substance to be tested on the NC membrane marking line, then add an appropriate amount of sample diluent to the sample pad, and let it stand for 20 minutes to observe the test results. The assembly of the reagent strips and the determination of the test results of the clinical samples are the same as in Example 1.

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Abstract

The invention discloses a rapid detective reagent strip of treponema pallidum immunoglobulin m (IgM) antibody. The rapid detective reagent strip of the treponema pallidum IgM antibody comprises an upper sampling pad (1), a colloidal gold pad (2), a NC film (3), an absorbing sampling pad (4) and a plastic plate (7). The colloidal gold pad (2) is marked with a T1 antibody and a TP fusion protein. An upper peridium man-resistant IgM mu chain monoclonal antibody of the NC film (3) forms a T line (5) and a sheep-resistant-mouse immunoglobulin G (IgG) antibody forms a C line (6). The upper sampling pad (1), the colloidal gold pad (2), the NC film (3) and the absorbing sampling pad (4) are pasted on the plastic plate (7) to form a detective reagent strip. Compared with the prior art, the rapid detective reagent strip of the treponema pallidum IgM antibody has the advantages of being high in sensitivity, strong in specificity and easy and convenient to operate. The rapid detective reagent strip of the treponema pallidum IgM antibody can provide an exact experimental basis for observation of curative effects after a clinical early stage diagnosis, an early treatment and an anti-luetic treatment.

Description

technical field [0001] The invention relates to a treponema pallidum IgM antibody detection reagent, in particular to a treponema pallidum IgM antibody rapid detection reagent strip and a preparation method thereof. Background technique [0002] Syphilis is a sexually transmitted disease caused by Treponema pallidum (TP), which is mainly transmitted through sexual behavior and blood, and can also be vertically transmitted from mother to child through the placenta. In recent years, the incidence of syphilis in my country has been increasing year by year. In 2009, the incidence of syphilis ranked third in the national statutory infectious disease epidemic report, an increase of 18.39% compared with 2008. Syphilis patients are the only source of transmission of syphilis, early diagnosis and early treatment of syphilis is a crucial link to control its spread. Treponema pallidum can invade various organs of the whole body, causing multiple organ lesions, dysfunction, tissue dest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 李洲许俊艳
Owner 天津中新科炬生物制药股份有限公司
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