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Multilayer tubular structural cell culture bracket as well as preparation method and use thereof

A cell culture, tubular structure technology, applied in the multi-layer tubular structure cell culture scaffold and its preparation, the field of three-dimensional tubular structure with single or multiple cells layered distribution, can solve the influence of the tubular structure, long culture time, impossible Realize the controllable distribution and growth of a variety of cells, and achieve the effect of obvious advantages

Inactive Publication Date: 2013-06-26
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing problems are: the cultivation time is long, and due to the need for manual crimping and the need to use O-rings to fix the tubular structure, there are human factors affecting the tubular structure
The polarity of cell growth in the tubular structure does not match that of real organs; it is even more impossible to achieve a controlled distribution of multiple cells on the material

Method used

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  • Multilayer tubular structural cell culture bracket as well as preparation method and use thereof
  • Multilayer tubular structural cell culture bracket as well as preparation method and use thereof
  • Multilayer tubular structural cell culture bracket as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The preparation of PDMS self-curling film (i.e., multi-layer tubular structure cell culture scaffold) is as follows:

[0058] (1) Preparation of polymer elastic film layer, i.e. PDMS thin film: Prepolymer and curing agent are prepared according to the ratio of 10:1 PDMS prepolymer reaction liquid, and spin-coated on the surface of smooth single crystal silicon wafer in the glue homogenizer, The rotating speed is 3000rpm, and the time is 30s. Place in a blast drying oven and bake at 150°C for 15 minutes, then take it out of the oven.

[0059] (2) Preparation of polymer immobilized layer: spin-coat the reaction solution of PDMS prepolymer (the same as the PDMS prepolymer in the above steps) on the surface of a clean glass slide in a homogenizer, the speed is 6000rpm, and the time is 60s . Put it in a blast drying oven and bake at 80°C for 4 minutes, then take it out of the oven. It is required that the PDMS prepolymer reaction solution on the surface of the glass slide ...

Embodiment 2

[0063] The preparation of the self-rolling film (that is, multi-layer tubular structure cell culture support) with porous structure, the specific steps are as follows:

[0064] (1) Preparation of a polymer elastic film layer with a porous structure, that is, a PDMS film: the first is the preparation of the template, the main process is photolithography, that is, using the characteristics of photoresist that can change properties under ultraviolet irradiation on a single crystal silicon wafer A columnar microarray is prepared, the columnar diameter is adjustable from 1 micron to 10 mm, and the height is adjustable from 0.1 micron to 1 mm. The photolithography template needs to be used after perfluorosilane treatment. The second is the preparation of porous PDMS film. The method is soft etching technology. The PDMS prepolymer reaction solution is spin-coated on the photolithographic template in a homogenizer. After heating for 5 minutes, take it out, and carefully peel off the ...

Embodiment 3

[0069] The preparation of the PDMS self-curling film (that is, a multilayer tubular structure cell culture support) with a microgroove structure in the polymer immobilization layer, the specific steps are as follows:

[0070] (1) Preparation of polymer elastic film layer, i.e. PDMS thin film: PDMS prepolymer and curing agent are prepared according to the ratio of 10:1 PDMS prepolymer reaction solution, and spin-coated on the surface of smooth single crystal silicon wafer in a glue homogenizer , the rotational speed is 2000rpm, and the time is 60s. Put it in a blast drying oven and bake at 150°C for 5 minutes, take it out of the oven, and peel off the PDMS film from the silicon wafer.

[0071](2) Preparation of a polymer fixed layer with a microgroove structure: first, the preparation of the template, the main process is photolithography, that is, using the characteristics of photoresist that can change properties under ultraviolet irradiation to prepare a raised cuboid array o...

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Abstract

The invention provides a multilayer tubular structural cell culture bracket as well as a preparation method and use thereof. The bracket comprises a macromolecular elastic thin film layer and a macromolecular fixed layer attached to the macromolecular elastic thin film layer. The macromolecular elastic thin film layer is elastic, so that the bracket is automatically crimped to a multilayer tubular structure. The preparation method of the cell culture bracket comprises the following steps of: preparation of the macromolecular elastic thin film layer; preparation of the macromolecular fixed layer; drawing the macromolecular elastic thin film layer and attaching the macromolecular elastic thin film layer to the macromolecular fixed layer; and forming the tubular structure. The use is that a method for distributing single / multiple cells in a lamellar manner in the multilayer tubular structural cell culture bracket is provided, and use for preparing a three-dimensional tubular structure with single / multiple cells distributed in a lamellar manner by the method. The bracket is used for simulating human organ with multiple cells distributed in the lamellar manner of blood vessel or intestinal tract, repairing diseased or damaged organs and being used as an in vitro study model.

Description

technical field [0001] The invention relates to a multilayer tubular structure cell culture support and its preparation method and application, specifically a method for distributing single or multiple cell layers in a multilayer tubular structure cell culture support and the single cell culture support prepared by the method or the use of a three-dimensional tubular structure with layered distribution of multiple cells, which belongs to the technical field of tissue engineering. Background technique [0002] The essence of tissue engineering is to use living cells to construct a new functional tissue. These cells are combined with extracellular matrix or scaffold materials in a certain way, and the cell matrix and scaffold materials guide the development of tissues. Using tissue engineering methods to construct artificial organs in vitro and then implant them into the body has a good prospect in the treatment of many diseases, especially in the repair of organs. Among them...

Claims

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Application Information

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IPC IPC(8): C12M3/00B32B27/08B32B7/12C12N5/071C12N5/078A61L27/40A61L27/50A61L27/56A61L31/12A61L31/14
Inventor 蒋兴宇靳钰袁博张伟
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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