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Preparation method for extracting cinobufagin and bufalin mixture from venenum bufonis

A technology for chinabufoni-based and bufalin, which is applied in the field of extracting high-purity chinabufoni-based and bufalin mixtures, and can solve the problems of low purity and the like

Inactive Publication Date: 2013-07-10
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the extracts of these two components in the prior art, the purity is generally very low

Method used

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  • Preparation method for extracting cinobufagin and bufalin mixture from venenum bufonis
  • Preparation method for extracting cinobufagin and bufalin mixture from venenum bufonis
  • Preparation method for extracting cinobufagin and bufalin mixture from venenum bufonis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Toad venom is crushed into a coarse powder that passes through a 40-mesh sieve, 95% ethanol is added to 30 times the amount of toad venom, heated and refluxed for 3 times, each extraction is 3 hours, the extract is filtered, and the ethanol is recovered from the filtrate until it has no ethanol smell. Ethanol extract A;

[0042] (2) In the extract A, add 5 times the amount of toad venom, silica gel with a particle size of 100-200 mesh, evaporate to dryness, and add the top of the chromatography column that has been filled with silica gel (the diameter-to-height ratio of the silica gel column is 2.5:13.5, and the loading capacity is 20 to 30 times the amount of toad venom in extract A), first use petroleum ether-acetone (9:1) mixed solvent as the eluent, elute, discard the eluent, and then use petroleum ether-acetone (8.5: 1.5) The mixed solvent is used as the eluent, and the eluent B is obtained when the components of the mixture of Cinobufacin and Bufafalin are co...

Embodiment 2

[0046] Toad venom is crushed into a fine powder that passes through a 40-mesh sieve, 80% ethanol is added to 40 times the amount of toad venom, heated and refluxed for 3 times, each extraction is 2 hours, the extract is filtered, and the ethanol is recovered from the filtrate until it has no ethanol smell, and the ethanol extract is obtained A;

[0047] (2) In the extract A, add 5 times the amount of toad venom, silica gel with a particle size of 100-200 mesh, evaporate to dryness, and add the top of the chromatography column that has been filled with silica gel (the diameter-to-height ratio of the silica gel column is 1:10, and the filling volume is 20 to 30 times the amount of toad venom in extract A), first use petroleum ether-acetone (9:1) mixed solvent as the eluent, elute, discard the eluent, and then use petroleum ether-acetone (8.5: 1.5) The mixed solvent is used as the eluent, and the eluent B is obtained when the components of the mixture of Cinobufacin and Bufafalin...

Embodiment 3

[0051] Toad venom is crushed into a fine powder that passes through a 20-mesh sieve, 70% ethanol is added to 20 times the amount of toad venom, heated and refluxed for extraction twice, each time for 4 hours, the extract is filtered, and the ethanol is recovered from the filtrate until it has no ethanol smell, and the ethanol extract is obtained A;

[0052](2) In the extract A, add 5 times the amount of toad venom, silica gel with a particle size of 100-200 mesh, evaporate to dryness, and add the top of the chromatography column that has been filled with silica gel (the diameter-to-height ratio of the silica gel column is 1:3, and the filling volume is 20 to 30 times the amount of toad venom in extract A), first use petroleum ether-acetone (9:1) mixed solvent as the eluent, elute, discard the eluent, and then use petroleum ether-acetone (8.5: 1.5) The mixed solvent is used as the eluent, and the eluent B is obtained when the components of the mixture of cinobufaction base and ...

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Abstract

The invention relates to a preparation method for extracting a cinobufagin and bufalin mixture from venenum bufonis. The preparation method is characterized by comprising the following steps of: smashing the venenum bufonis into medium coarse powder, and then adding 70%-95% of ethanol for extraction; and carrying out silica gel column chromatography separation on an extracting solution to obtain the high-purity cinobufagin and bufalin mixture, wherein the content of the cinobufagin and bufalin mixture achieves more than 80%. The total content of the cinobufagin and bufalin mixture extracted by adopting the preparation method disclosed by the invention achieves more than 80%.

Description

technical field [0001] The invention relates to a method for extracting a mixture of high-purity cinobufotoxin base and bufalin from traditional Chinese medicine toad venom, belonging to the technical field of traditional Chinese medicine. Background technique [0002] Toad Su, a traditional Chinese medicine, is derived from the dry secretion of Bufo bufo gargarizans Cantor or Bufo melanostictus Schneider, which are Bufo bufo animals. Xin, temperature; poisonous. Guixinjing has detoxification, pain relieving, resuscitating and refreshing functions, poisonous. Used for carbuncle and furuncle, sore throat, sunstroke, coma, abdominal pain, vomiting and diarrhea due to fever. Toad venom has been paid attention to at home and abroad because modern pharmacological studies have shown that it has anti-tumor, anti-pathogenic microorganism, and cardiotonic effects. It is the active ingredient with higher content in toad venom. [0003] Due to different origins, different processin...

Claims

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Application Information

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IPC IPC(8): A61K31/585
Inventor 林桂涛张超邓华亮
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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