Laser-induced fluorescence spectrum detection device

Abstract

The invention discloses a laser-induced fluorescence spectrum detection device, relating to the technical field of spectrum detection and aiming at solving the technical problems of low collecting efficiency and high demand on the form of a fluorescent material sample in the prior art. The laser-induced fluorescence spectrum detection device comprises a light source, a fluorescent probe and a grating spectrometer, wherein the fluorescent probe comprises a collimating lens, a narrow-band pass filter, an exciting light reflector, a dichroism light filter, a side pass filter and two focusing lenses; each part forms an exciting light path and a signal detection light path; the exciting light path starts from the light source, then sequentially passes through the collimating lens, the narrow-band pass filter, the exciting light reflector and the dichroism light filter, and then passes through the first focusing lens; the signal detection light path starts from the first focusing lens, then sequentially passes through the dichroism light filter, the side pass filter and the second focusing lens, and then enters the grating spectrometer. The laser-induced fluorescence spectrum detection device is especially suitable for detecting the fluorescence spectrum of fluorescent materials such as a quantum dot, water quality and jewel.

Description

technical field [0001] The invention relates to spectrum detection technology, in particular to the technology of a laser-induced fluorescence spectrum detection device. Background technique [0002] When ultraviolet light or shorter-wavelength visible light hits certain substances, these substances emit visible light of various colors and varying intensities, and when the light source stops shining, this light disappears. The light induced by the excitation light is called fluorescence, and the substance that can emit fluorescence is called fluorescent substance. [0003] It can be known from the principle of fluorescence that the molecular fluorescence spectrum has nothing to do with the wavelength of the excitation light source, but only with the energy level structure of the fluorescent substance itself. Therefore, the fluorescent substance can be qualitatively analyzed and identified according to the fluorescence spectrum generated by the fluorescent substance. It has ...

AI Technical Summary

Problems solved by technology

[0006] 1) The monochromator needs to be equipped with a driving component that drives the grating to rotate, so its volume and power consumption are large, resulting in the volume and power consumption of the entire device are too large, and the time period for obtaining the fluorescence spectrum is long, and the fluorescence spectrum cannot be realized. Real-time collection of spectra, so some transient characteristics of fluorescent signals cannot be analyzed, and spectral repeatability is poor;

[0007] 2) The low intensity and poor quality of the light beam generated by the xenon lamp makes the number of molecules of the fluorescent substance excited to the excited state less, and the fluorescence intensity produced by the fluorescent substance is also weak, so the detection sensitivity of the existing fluorescence spectrum detection device is low;

[0008] 3) Existing fluorescent probes irradiate fluorescent substances from an angle of 0 degrees and receive fluorescent signals from a direction of 45 degrees. This collection method has low fluorescence collection efficiency and can collect less fluorescence. The shape requirements of the fluorescent material sample are relatively high, and the shape of the fluorescent material sample does not meet the requirements, which will affect the collection of fluorescent signals. The existing fluorescent probes are currently mainly used for liquid fluorescent material samples, and their use scenarios are mainly laboratories.

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