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Method for reducing background of vector in biological reaction

A biological reaction and carrier technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high background value and poor signal strength, and achieve the effect of reducing background, low cost and simple operation

Inactive Publication Date: 2015-04-15
王颖颖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection often encounters the problems of high background value and poor signal strength.

Method used

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  • Method for reducing background of vector in biological reaction
  • Method for reducing background of vector in biological reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of gene chip

[0032] (1) NC membrane staining:

[0033] ① Soak the NC film in 0.2% Tween-20 solution, soak it at room temperature for 3 hours, and then take it out; immerse the soaked film in dye, dye at 85°C for 1 hour, and shake gently every 5 minutes during the period; ② Pour off the dye solution , rinse 3 times with deionized water;

[0034] ③ Soak the rinsed membrane in deionized water at room temperature for more than 12 hours;

[0035] ④Pour out the deionized water and rinse twice with deionized water, add washing solution (0.2M PBS-T), and wash at 50°C for 5 hours;

[0036] ⑤ Pour out the washing solution and rinse with deionized water 3 times;

[0037] ⑥ Add deionized water, 50 ℃, soak for 1 hour, and repeat again;

[0038] ⑦Take out the washed membrane, rinse it with deionized water, and dry it at 37°C for later use.

[0039] (2) Coating: Coating different nucleic acid probes on the surface of nitrocellulose membrane according to certain requ...

Embodiment 2

[0045] Preparation of immunochips (fluorescence method)

[0046] (1) Coating: Coating different biological substances on the surface of cellulose acetate membrane with certain requirements.

[0047] (2) After the assembly package is completed, the cellulose acetate membrane containing biological substances is assembled into an immunochip.

[0048](3) Preparation of coloring solution: 0.5 g of aniline blue is dissolved in 100 ml of distilled water, boiled and cooled, then 1 g of orange G and 1.5 g of acid fuchsin are added, and the pH is corrected to 1.9 with HCC. use.

[0049] (4) Blocking: Add 1.0% coloring solution to the blocking solution, and seal the immunochip with 100ul of blocking solution per well.

[0050] (5) Drying: After sealing the immunochip, dry it in a drying oven.

[0051] (6) Packaging: The immunochip is sealed and packaged after drying.

Embodiment 3

[0053] Preparation of biochips

[0054] (1) Coating: Coating different kinds of biological substances on the surface of nitrocellulose membrane with certain requirements.

[0055] (2) After the assembly package is completed, the nitrocellulose membrane containing biological substances is assembled into a biochip.

[0056] (3) Blocking: Add 0.4% methyl violet to the blocking solution, and seal the biochip with 100 ul of blocking solution per well.

[0057] (4) Drying: After sealing the biochip, dry it in a drying oven.

[0058] (5) Packaging: The biochip is sealed and packaged after drying.

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Abstract

The present invention relates to a method for reducing background of a vector in a biological reaction, and belongs to the technical field of biological detection. According to the method for reducing the background of the vector in the biological reaction, the used biological reaction vector is a colored vector formed by coloring process. The biological reaction vector is a vector which can be combined with coated biological materials. The method of the present invention can significantly reduce the background during biological reaction, and improve the detection sensitivity, and has the advantages of low cost and easiness to operate.

Description

technical field [0001] The invention relates to a method for reducing the background of a carrier in a biological reaction, and belongs to the technical field of biological detection. Background technique [0002] At present, biological response systems are widely used in various research fields of life sciences such as genome research, medical research and pharmaceutical research, and are gradually applied to new drug development, clinical diagnosis, health prediction, disease treatment, judicial identification, military protection, food hygiene supervision, It penetrates into various fields such as commercial inspection and quarantine, environmental monitoring and control, and selection and optimization of agricultural, forestry, animal husbandry and fishery crops. Especially in the aspect of disease detection and diagnosis, biological response system has unique advantages. Multiple diseases or indicators can be detected on multiple patients at the same time on one carrie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543
Inventor 王颖颖
Owner 王颖颖