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Application of tea polyphenol in biological prevention and control of aflatoxin

An aflatoxin, biological control technology, applied in aflatoxin biological control preparations, the application field of tea polyphenols in aflatoxin biological control, can solve the problems of poor persistence, application limitations, etc., to achieve safety High performance, high feasibility and stable effect

Active Publication Date: 2013-07-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant extracts mainly include litsea cubeba essential oil, thyme oil, hippocampus tooth essential oil, rose essential oil, etc. These essential oils are volatile substances, which have a strong inhibitory effect on the growth of Aspergillus flavus and the production of toxins, but the persistence is poor ; Among microorganisms, lactic acid bacteria, bacillus, yellow yellow bacillus, myxobacteria, pseudomonas, Ralstia, Burkholderia, some yeasts and a small amount of fungi all have the ability to inhibit the growth and toxin production of Aspergillus flavus, But in the storage period of agricultural products or food processing, the application of these microorganisms is limited

Method used

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  • Application of tea polyphenol in biological prevention and control of aflatoxin
  • Application of tea polyphenol in biological prevention and control of aflatoxin
  • Application of tea polyphenol in biological prevention and control of aflatoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The plate antibacterial experiment confirmed that tea polyphenols had a significant effect on the growth rate of Aspergillus flavus As3.4408CGMCC3.4408 (China Microorganism Culture Collection Center) strain colony growth rate. The experimental process is as follows:

[0023] (1) Make PDA medium containing tea polyphenols: Dissolve tea polyphenols (the purity of tea polyphenols is 80%, Sichuan Fuzhengyuan Biotechnology Co., Ltd.) v) (tea polyphenols 5g is dissolved in 1L distilled water to obtain a solution with a concentration of 0.5% (m / v)) and 1% (m / v) tea polyphenols solution (tea polyphenols 10g is dissolved in 1L distilled water to obtain a concentration of 1 % (m / v) solution), then replace distilled water with these two kinds of tea polyphenol solutions respectively, prepare PDA solid medium according to the conventional method, pour into the sterilizing plate that diameter is 9cm after sterilization, obtain the tea polyphenol-containing PDA tablet. Prepare the ...

Embodiment 2

[0033] In this example, tea polyphenols are used to inhibit the biosynthesis of aflatoxins in several agricultural products, that is, inoculate Aspergillus aflatoxin As3.4408 on peanuts, rice, soybeans, walnut kernels, almonds, and corn seeds to study tea polyphenols. Inhibitory effect on aflatoxin biosynthesis of several agricultural products during storage.

[0034] experimental method:

[0035] The experimental materials and reagents are as follows:

[0036] Commercially available peanuts, rice, soybeans, walnut kernels, almonds, and corn are each 1kg;

[0037] Aflatoxin producing strain: Aspergillus flavus As3.4408CGMCC3.4408 (China Microorganism Culture Collection Center);

[0038] Tea polyphenols (mass concentration is 80%, Sichuan Fuzhengyuan Biotechnology Co., Ltd.);

[0039] The operation steps are as follows:

[0040] (1) Inoculation culture:

[0041] 1) Strain activation: Inoculate the As3.4408 strain of Aspergillus flavus on the slant of the Chapei culture mediu...

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Abstract

The invention belongs to food biotechnology field, and discloses an application of tea polyphenol in biological prevention and control of aflatoxin, tea polyphenol can be used for inhibiting the growth of producing bacteria of aflatoxin and biosynthesis of aflatoxin, and the application is a new application for tea polyphenol. The researches show that the tea polyphenol has obvious effect for inhibiting the growth of producing bacteria of aflatoxin, and the inhibition effect has concentration-dependent relation. The tea polyphenol also has obvious effect for inhibiting the biosynthesis of aflatoxin, the inhibition ratio of 0.2% of tea polyphenol on aflatoxin is 21%-59%, and the inhibition ratio of 1% of tea polyphenol on aflatoxin is 49%-81%, and the tea polyphenol can be used for prevention and control of aflatoxin in a storage process of food and feed.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and relates to a biological control preparation of aflatoxin, in particular, relates to the application of tea polyphenols in the biological control of aflatoxin, and is a method for inhibiting the production of aflatoxin by using tea polyphenols. Bacterial growth and its method of aflatoxin biosynthesis. Background technique [0002] Aflatoxins (AFs for short) is a general term for a class of dihydrofuranoxaline derivatives with similar structures. According to their molecular structures, there are currently at least 17 different structures including B1, B2, G1, and G2. of aflatoxins were identified. Aspergillus flavus and A.parasiticus are the two main fungi that produce aflatoxin, but in recent years, A.nomius, A.tamarii, A.bombycis, A.pseudotamarii etc. can also produce AFs. Toxicity test proves that B1 is the most toxic, followed by: M1>G1>B2>M2>G2. The main chemical charact...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P3/00A23L3/3544A23K1/00
Inventor 方祥周建良佘燕珊鲁素珍叶宏襄刘威
Owner SOUTH CHINA AGRI UNIV
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