Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting onion osteogenic peptide

An extraction method and technology of osteogenic peptides, which are applied in the fields of peptides, bone diseases, drug combinations, etc., can solve the problems such as the extraction method of onion osteogenic peptides, etc., and achieve the effect of high-efficiency extraction.

Inactive Publication Date: 2013-07-24
辽宁琦润生物科技有限公司
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the invention patents on the extraction method of onion active ingredients involve the extraction of onion volatile oils, and there is no patent related to the extraction method of onion osteogenic peptides.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting onion osteogenic peptide
  • Method for extracting onion osteogenic peptide
  • Method for extracting onion osteogenic peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The schematic diagram of the extraction process of onion osteogenic peptides is as follows figure 1 As shown, the extraction steps are as follows:

[0034] A), take 50kg of fresh purple onion, peel and wash, cut into pieces, dry under the condition of lower than 80 ℃, pulverize and pass through a 60-mesh sieve;

[0035] B), soak with 50L of 75% ethanol (volume concentration) at room temperature for 2 weeks, and use a rotary evaporator to recover ethanol in a water bath below 80°C to obtain 10kg of extract;

[0036] C), dissolve the extract with 25L of pure water, then extract 3 times with 25L of dichloromethane, combine the three aqueous layer solutions, and use a rotary evaporator to recover the dichloromethane in a water bath below 80 ° C to obtain a crude onion polypeptide Extract 1 total 6kg;

[0037] D), put the crude extract 1 of onion polypeptide on the D-101 macroporous resin chromatographic column, and elute with 50% ethanol (volume concentration, the same be...

Embodiment 2

[0046] The schematic diagram of the extraction process of onion osteogenic peptides is as follows figure 1 As shown, the extraction steps are as follows:

[0047] A), take 50kg of fresh purple onion, peel and wash, cut into pieces, dry under the condition of lower than 60 ℃, pulverize and pass through a 60-mesh sieve;

[0048] B), soak with 50L of 75% ethanol at room temperature for 2 weeks, and use a rotary evaporator to recover ethanol in a water bath below 80°C to obtain 10.3kg of extract;

[0049] C), dissolve the extract with 25L of pure water, then extract 3 times with 25L of chloroform, combine the three aqueous layer solutions, and use a rotary evaporator to recover chloroform in a water bath below 80 ° C to obtain onion polypeptide crude extract 1 total 6.1kg;

[0050] D), put the crude extract 1 of onion polypeptide on the D-101 macroporous resin chromatographic column, eluted with 50% ethanol until the column is colorless (about 30L of 50% ethanol), and the eluent...

Embodiment 3

[0057] The schematic diagram of the extraction process of onion osteogenic peptides is as follows figure 1 As shown, the extraction steps are as follows:

[0058] A), take 50kg of fresh purple onion, peel and wash, cut into pieces, dry under the condition of lower than 80 ℃, pulverize and pass through a 60-mesh sieve;

[0059] B), soak with 50L of 75% ethanol at room temperature for 2 weeks, and use a rotary evaporator to recover ethanol in a water bath below 80°C to obtain 9.8kg of extract;

[0060] C), dissolve the extract with 25L of pure water, then extract 3 times with 25L of dichloromethane, combine the three aqueous layer solutions, and use a rotary evaporator to recover the dichloromethane in a water bath below 80 ° C to obtain a crude onion polypeptide Extract 1 total 5.9kg;

[0061] D), put the crude extract 1 of onion polypeptide on the AB-8 macroporous resin chromatographic column, and elute with 50% ethanol until the column is colorless (about 30L of 50% ethanol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention aims to provide a method for extracting onion osteogenic peptide-glutathione capable of preventing osteoporosis. The method comprises the following steps: preparing onion polypeptide crude extract by employing a low-temperature alcohol extraction process, sequentially separating and purifying the extract through macroporous resin chromatography, silica gel chromatography and Sephadex LH20 chromatography, obtaining a polypeptide refined extract containing the onion osteogenic peptide, and performing freeze drying to prepare dry powder for saving. The method does not produce poisonous gas and lots of industrial residues and is pollution-free; low-temperature operation is performed in the whole process, the target components are not damaged, and the onion osteogenic peptide can be efficiently extracted at high quality.

Description

technical field [0001] The invention belongs to the technical field of health care product production, and in particular relates to a method for extracting onion osteogenic peptides, which is used for extracting onion osteogenic peptides with high efficiency, high quality and no pollution, and the obtained onion osteogenic peptides can effectively improve osteoporosis symptoms and prevent Can prevent the development of osteoporosis. Background technique [0002] Osteoporosis is a systemic bone disease characterized by decreased bone mass and degeneration of bone microstructure, resulting in increased bone fragility and susceptibility to fractures. The World Health Organization has issued a warning: Osteoporosis has become the second major disease threatening human health after cardiovascular disease. According to the survey, there are about 90 million osteoporosis patients (accounting for 60%) among the 150 million elderly people over 60 years old in my country. The inciden...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/037C07K1/16A61P19/10
Inventor 张英杰高劲松
Owner 辽宁琦润生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com