Method for detection of HIV-1 nucleoside inhibitor drug resistance mutation and kit

Abstract

The invention relates to a method for detection of HIV-1 (human immunodeficiency virus-1) nucleoside inhibitor drug resistance mutation and a kit thereof. Specifically, the invention discloses a specific probe for detection of human immunodeficiency virus nucleoside reverse transcriptase inhibitor drug resistance mutation, and the probe includes at least one of: a probe for detection of reverse transcriptase gene M41L mutation, a probe for detection of reverse transcriptase gene A62V mutation, a probe for detection of reverse transcriptase gene K65R mutation, a probe for detection of reverse transcriptase gene D67N mutation, a probe for detection of reverse transcriptase gene 69INS insertion mutation, a probe for detection of reverse transcriptase gene K70R mutation, a probe for detection of reverse transcriptase gene L74V mutation, a probe for detection of reverse transcriptase gene V75I mutation, a probe for detection of reverse transcriptase gene F77L mutation, a probe for detection of reverse transcriptase gene Y115F mutation, a probe for detection of reverse transcriptase gene F116Y mutation, and the like.

Description

technical field [0001] The invention relates to a method for detecting drug-resistant mutations of HIV-1 virus nucleoside inhibitors and a kit thereof. Background technique [0002] 1. Mechanism of nucleoside and nucleoside analog drugs: [0003] Human immunodeficiency virus (HIV) is a retrovirus and is divided into HIV-1 and HIV-2. AIDS patients in most parts of the world are infected by HIV-1 virus, and existing drugs mainly inhibit the replication of HIV-1 virus. The clinical course of HIV infection has changed significantly since the introduction of a new antiviral regimen, HAART in combination with reverse transcriptase inhibitors and protease inhibitors, in 1995. These drugs allow the immune system to rebuild its CD by blocking viral replication 4 + T cell repertoire and restore immunity to pathogens, significantly reducing the morbidity and mortality of HIV / AIDS-related diseases. Up to now, the U.S. FDA has approved a total of 26 antiviral drugs in 4 categories f...

AI Technical Summary

Problems solved by technology

Phenotypic drug resistance is the gold standard for drug resistance detection. It directly provides drug resistance and can be quantified, but the disadvantages are also obvious, namely high technical requirements, time-consuming, and high cost. Therefore, gene is widely used in clinical practice internationally. type resistance detection

[0015] Further application limitations of genotypic and phenotypic testing include lack of quality assurance for existing testing methods; high cost of testing; insensitivity to parvovirus specimens; There are detection methods that may not detect

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