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Chitosan polyacrylamide hydrogel base material and preparation method thereof

A polyacrylamide and base material technology, applied in biochemical equipment and methods, tissue culture, microorganisms, etc., can solve problems such as complex research, multiple influencing factors, and single impact

Active Publication Date: 2013-07-31
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Disadvantages of the prior art: use polyacrylamide hydrogel as the substrate material with variable hardness to carry out cell culture research on the influence of different soft and hard substrates on cell elasticity and cell physiology, but the growth of cells on the substrate of polyacrylamide hydrogel requires expression Substances that are conducive to cell attachment, such as collagen, laminin, fibronectin, gelatin, polylysine, and peptides, have too many influencing factors, and the research is complicated. It is impossible to study the influence of substrate stiffness on cells alone.

Method used

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  • Chitosan polyacrylamide hydrogel base material and preparation method thereof
  • Chitosan polyacrylamide hydrogel base material and preparation method thereof
  • Chitosan polyacrylamide hydrogel base material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A chitosan polyacrylamide hydrogel base material is composed of the following components in parts by volume:

[0064] 30% (w / v) acrylamide solution: 1.25 parts;

[0065] 1% (w / v) methylene bisacrylamide solution: 0.5 parts;

[0066] h 2 O: 3.25 copies;

[0067] 0.03 parts of ammonium persulfate solution;

[0068] 0.02 part of tetramethylethylenediamine;

[0069] 0.5 parts of 1% (w / v) chitosan solution.

[0070] Prepare 1% (w / v) chitosan solution: add 0.5g chitosan to 50mL 1% acetic acid solution, stir until dissolved at 37°C, and after standing for 24 hours, a 1% (w / v) chitosan solution is obtained .

[0071] A preparation method of chitosan polyacrylamide hydrogel base material, comprising the following steps:

[0072] (1) Mixing and stirring: add 30% (w / v) acrylamide solution, 1% (w / v) methylenebisacrylamide solution, ammonium persulfate solution, tetramethylethylenediamine, 1 % (w / v) chitosan solution is mixed evenly, then added to the centrifuge tube, and st...

Embodiment 2

[0076] A chitosan polyacrylamide hydrogel base material is composed of the following components in parts by volume:

[0077] 30% (w / v) acrylamide solution: 1.25 parts;

[0078] 1% (w / v) methylene bisacrylamide solution: 3 parts;

[0079] h 2 O: 0.75 parts;

[0080] 0.03 parts of ammonium persulfate solution;

[0081] 0.02 part of tetramethylethylenediamine;

[0082] 0.5 parts of 1% (w / v) chitosan solution.

[0083] Prepare 1% (w / v) chitosan solution: add 0.5g chitosan to 50mL 1% acetic acid solution, stir until dissolved at 37°C, and after standing for 24 hours, a 1% (w / v) chitosan solution is obtained .

[0084] A preparation method of chitosan polyacrylamide hydrogel base material, comprising the following steps:

[0085] (1) Mixing and stirring: add 30% (w / v) acrylamide solution, 1% (w / v) methylenebisacrylamide solution, ammonium persulfate solution, tetramethylethylenediamine, 1 % (w / v) chitosan solution is mixed evenly, then added to the centrifuge tube, and stirr...

Embodiment 3

[0089] A chitosan polyacrylamide hydrogel base material is composed of the following components in parts by volume:

[0090] 30% (w / v) acrylamide solution: 1.25 parts;

[0091] 1% (w / v) methylene bisacrylamide solution: 1.5 parts;

[0092] h 2 O: 2.25 copies;

[0093] 0.03 parts of ammonium persulfate solution;

[0094] 0.02 part of tetramethylethylenediamine;

[0095] 0.5 parts of 1% (w / v) chitosan solution.

[0096] Prepare 1% (w / v) chitosan solution: add 0.5g chitosan to 50mL 1% acetic acid solution, stir until dissolved at 37°C, and after standing for 24 hours, a 1% (w / v) chitosan solution is obtained .

[0097]A preparation method of chitosan polyacrylamide hydrogel base material, comprising the following steps:

[0098] (1) Mixing and stirring: add 30% (w / v) acrylamide solution, 1% (w / v) methylenebisacrylamide solution, ammonium persulfate solution, tetramethylethylenediamine, 1 % (w / v) chitosan solution is mixed evenly, then added to the centrifuge tube, and sti...

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Abstract

The invention discloses a chitosan polyacrylamide hydrogel base material and a preparation method thereof. The chitosan polyacrylamide hydrogel base material is composed of an acrylamide solution, a methylene diacrylamide solution, H2O, an ammonium persulfate solution, tetramethyl ethylenediamine and a chitosan solution. The preparation method of the chitosan polyacrylamide hydrogel base material comprises a step of mixing and stirring, a step of transferring to a glass clamping plate for reacting, and a step of immersion. The chitosan polyacrylamide hydrogel base material has a simple technology and allows hydrogels having different hardnesses to be obtained, and the obtained hydrogels have the advantages of non-toxicity, easy adherence growth of cells, reduction of the reaching complexity of the cytomechanics, benefiting for the researches on the mechanical properties of the cells, and reduction of the researching cost.

Description

technical field [0001] The invention belongs to the field of hydrogel technology, and in particular relates to a chitosan polyacrylamide hydrogel base material and a preparation method thereof. Background technique [0002] As the basic unit of the structure and function of organisms, cells can sense and respond to external physical, chemical and biological stimuli. The substrate stiffness of cell growth is an important mechanical research parameter, and it is also a hotspot in the field of cell mechanics. Studies by Engler et al. have shown that stem cells are very sensitive to tissue elasticity, and can differentiate (specialize) into a certain lineage and produce corresponding phenotypes with changes in tissue elasticity index. The soft matrix similar to brain tissue has a "nerve-oriented" induction function, the slightly harder matrix of muscle tissue has a "muscle-oriented" induction function, and the hardness of cartilage has a "bone-oriented" induction function. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L33/26C08L5/08C08F220/56C08J3/075C12N5/00
Inventor 叶志义吴贵生刘良臣
Owner CHONGQING UNIV
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