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Tissue culture rapid propagation method of Dendrobium officinale

A technology of rapid propagation of Dendrobium officinale, tissue culture, applied in the field of agriculture, can solve the problems that the number of seedlings cultivated in a large area cannot be satisfied, and the reproduction coefficient is low

Active Publication Date: 2013-08-07
浙江健九鹤药业集团有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The traditional asexual reproduction method has a low reproduction coefficient, and the number of seedlings cultivated in a large area cannot be satisfied

Method used

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Experimental program
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Embodiment 1

[0012] A method for tissue culture rapid propagation of dendrobium officinale, the method comprising:

[0013] 1) Select the annual stem segments of healthy Dendrobium officinale plants that grow vigorously and have no diseases, and put each 2 nodes into a 500mL beaker. It is better to float the stem section of Dendrobium officinale in water, then put it on the ultra-clean workbench in the aseptic room, remove the bract leaves of the axillary buds with the stem section, and sterilize;

[0014] 2) Weigh 3,000 grams of corn flour, 3,000 grams of wheat bran, 850 grams of sawdust, 750 grams of white granulated sugar, 30 grams of licorice, and 35 grams of Gansui, mix them evenly, add 10,000 grams of water and stir and mix to prepare a culture medium;

[0015] 3) Put the material in 1) into the culture medium in 2) and cultivate for 10 days, the culture conditions are temperature 26°C, light time 12h, and light intensity 2000Lx;

[0016] 4) When the stem section grows to 3-4, cut t...

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Abstract

The invention relates to a tissue culture rapid propagation method of Dendrobium officinale, which comprises the following steps: 1) sterilizing; 2) weighing 3000g of corn flour, 3000g of wheat bran, 850g of sawdust, 750g of white granulated sugar, 30g of licorice root and 35g of euphorbia kansui, evenly mixing, adding 10000g of water, and mixing to prepare a culture medium; 3) culturing the material in the step 1) in the culture medium in the step 2) for 10 days, wherein the culture conditions are as follows: the temperature is 26 DEG C, the illumination time is 12 hours, and the intensity of illumination is 2000 Lx; and 4) when the material grows to 3-4 stem segments, cutting the stem segment with buds, and removing the leaves to obtain an explant, and carrying out original corm induced culture, proliferation and differentiation in the culture medium prepared in the step 2) to obtain the Dendrobium officinale seedling. The method provided by the invention is convenient and practical.

Description

technical field [0001] The invention relates to the field of agriculture, in particular, the invention relates to a method for tissue culture and rapid propagation of Dendrobium officinale. Background technique [0002] Dendrobium officinale (Deudrobium officiuale) is a perennial epiphytic herb of the Orchidaceae Dendrobium genus. Stomach qi, long muscles, improving intelligence and relieving shock, committing suicide and prolonging life"; folks call it "life-saving celestial grass". It is mostly distributed on the semi-shady rocks in the mountains with an altitude of nearly 1,000 meters, and the long-term excavation has made the resources of wild Dendrobium candidum gradually exhausted [4-5]. Because the seeds have no endosperm and need to be combined with symbiotic bacteria to germinate normally, the cultivation of seedlings is difficult. The traditional asexual reproduction method has a low reproduction coefficient, and the number of seedlings cultivated in a large area...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 姚国祥
Owner 浙江健九鹤药业集团有限公司
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