Method for measuring combined-state aroma substances in cereal raw materials for food brewage
A technology of aroma substances and combined state, which is applied in the field of determination of aroma substances of grain raw materials for food brewing, can solve the problems that dry raw materials cannot be squeezed, and are not suitable for extraction of dry raw materials, so as to prevent decomposition, improve quality, and enrich The effect of the study
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Embodiment 1
[0022] Example 1: Extraction of Precursors of Bound Aroma Substances in Sorghum for Purification
[0023] Dry the sorghum and crush it, take 10g into a conical flask, add 20mL of acetonitrile / ethanol (v / v3:7), soak at room temperature for 12h, ultrasonicate for 0.5h, put the extract in a 50mL centrifuge tube, Centrifuge at 8000rpm for 15min at 4°C, save the supernatant, soak the residue in 20mL of acetonitrile / methanol (v / v3:7) for 12h, sonicate for 0.5h, put the extract in a 50mL centrifuge tube, and store at 4°C Centrifuge at 8000rpm for 15min, combine the obtained supernatant with the supernatant obtained in the previous step, put them in a 500mL rotary evaporator, evaporate to dryness under reduced pressure on a rotary evaporator, and wash with 100mL citric acid-phosphate buffer ( pH 7) was dissolved, then the solution was placed in a 250mL separatory funnel, and extracted 3 times with 20mL redistilled diethyl ether / pentane / dichloromethane (v / v / v: 1 / 1 / 3) to remove the vola...
Embodiment 2
[0024] Example 2: Extraction and Purification of Precursors of Combined Aroma Substances in Pea
[0025] Dry the peas and crush them, take 10g into a conical flask, add 20mL of acetone / ethanol (v / v3:7), soak at room temperature for 12h, ultrasonicate for 0.5h, put the extract in a 50mL centrifuge tube, Afterwards, centrifuge at 8000rpm for 15min at 4°C, save the supernatant, soak the residue in 20mL acetone / acetonitrile (v / v3:7) for 12h, sonicate for 0.5h, put the extract in a 50mL centrifuge tube, Centrifuge at 8000rpm for 15min at ℃, combine the obtained supernatant with the supernatant obtained in the previous step, put them in a 500mL rotary evaporator, evaporate to dryness under reduced pressure on a rotary evaporator, and wash with 100mL citric acid-phosphate buffer (pH5) was dissolved, then the solution was placed in a 250mL separatory funnel, and extracted three times with 20mL redistilled diethyl ether / pentane / ethyl acetate (v / v / v: 1 / 1 / 3) to remove volatile Sexual ar...
Embodiment 3
[0026] Example 3: Physicochemical treatment to detect bound aroma substances in corn
[0027] Dry the corn and crush it, take 10g into a conical flask, add 20mL of acetonitrile / ethanol (v / v3:7), soak at room temperature for 12h, and after ultrasonication for 0.5h, put the extract in a 50mL centrifuge tube, Then centrifuge at 8000rpm for 15min at 4°C, save the supernatant, soak the residue in 20mL acetonitrile / methanol (v / v3:7) for 12h, sonicate for 0.5h, put the extract in a 50mL centrifuge tube, Centrifuge at 8000rpm for 15min at ℃, combine the obtained supernatant with the supernatant obtained in the previous step, put them in a 500mL rotary evaporator, evaporate to dryness under reduced pressure on a rotary evaporator, and wash with 100mL citric acid-phosphate buffer (pH5) was dissolved, then the solution was placed in a 250mL separatory funnel, and extracted 3 times with 20mL redistilled diethyl ether / pentane / dichloromethane (v / v / v: 1 / 1 / 3) to remove volatile Sexual aroma ...
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