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Biological active microsphere and preparation method thereof

A biologically active and microsphere technology, applied in the field of biomedical material preparation, can solve the problems of cumbersome preparation of microemulsions, unfavorable industrial production, and complicated preparation methods, and achieve excellent fluidity, adjustable particle size, and simple preparation process Effect

Active Publication Date: 2013-08-14
佛山今兰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing preparation methods of bioactive glass microspheres cannot meet this demand, or there are defects such as the preparation method is too complicated.
[0003] Chinese Patent Publication No. CN 101215153A, invented a method for synthesizing ultrafine biological glass powder. The average particle size of the particles is 1 μm, but the particles are irregular in shape. The post-treatment process includes mechanical ball milling and freezing treatment, which is cumbersome.
Chinese Patent Publication No. CN 1843994A, invented a method for biomimetic preparation of living active glass nanoparticles, the particle size is 20nm-50nm, and the particle shape is spherical, but the preparation method uses a large amount of organic solvents, which is easy to cause bodily harm , and the process of preparing the microemulsion is too complicated, which is not conducive to industrial production

Method used

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  • Biological active microsphere and preparation method thereof
  • Biological active microsphere and preparation method thereof
  • Biological active microsphere and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] (1) Dissolve 0.2 gram of citric acid in 30 milliliters of absolute ethanol in the aqueous solution, the mol ratio of citric acid and tetraethyl orthosilicate is 0.017, stir magnetically for 10 minutes and mix well, then add 1.04g polyethylene glycol 6000 , continue stirring for 1 hour to form solution A;

[0035] (2) Add 12.5 grams of tetraethyl orthosilicate (TEOS), 1.45 grams of triethyl phosphate (TEP), and 8.501 grams of calcium nitrate tetrahydrate to the solution A obtained in step (1) in order. A raw material is fully hydrolyzed at an interval of 30 minutes, and sol B is formed after being fully stirred evenly;

[0036] (3) Put the sol B obtained in step (2) into a sealed 50ml plastic centrifuge tube and age at 60°C for 24 hours to obtain a wet gel;

[0037] (4) Washing the wet gel obtained in step (3) with deionized water and absolute ethanol three times to remove impurities, drying at 37°C for 24 hours, and then freeze-drying for 24 hours to obtain a xerogel; ...

Embodiment 2

[0041] (1) Dissolve 0.2 gram of citric acid in the aqueous solution of 30 milliliters of absolute ethanol, the mol ratio of citric acid and tetraethyl orthosilicate is 0.017, stir magnetically for 10 minutes and mix well, then add 1.25 gram of polyethylene glycol 4000, Stirring was continued for 1 hour to form solution A;

[0042] (2) Add 12.5 grams of tetraethyl orthosilicate (TEOS), 1.45 grams of triethyl phosphate (TEP), and 8.051 grams of calcium nitrate tetrahydrate to the solution A obtained in step (1) in sequence. A raw material is fully hydrolyzed at an interval of 30 minutes, and sol B is formed after being fully stirred evenly;

[0043] (3) Put the sol B obtained in step (2) into a sealed 50ml plastic centrifuge tube and age at 60° C. for 24 hours;

[0044] (4) The wet gel obtained in step (3) was washed 3 times with deionized water and absolute ethanol respectively;

[0045] (5) drying the wet gel in step (4) at 37° C. for 24 hours, and then freeze-drying for 24 ...

Embodiment 3

[0049](1) Dissolve 0.88 milliliter, 2 mol / liter hydrochloric acid in 30 milliliters of absolute ethanol aqueous solution, the molar ratio of hydrochloric acid to tetraethyl orthosilicate is 0.025, stir magnetically for 10 minutes and mix well, then add 1.82 grams of polyethylene Diol 10000, continue stirring for 1 hour to form solution A;

[0050] (2) Add 14.58 grams of tetraethyl orthosilicate (TEOS), 1.45 grams of triethyl phosphate (TEP), and 6.202 grams of calcium nitrate tetrahydrate to the solution A obtained in step (1) in order. A raw material is fully hydrolyzed at an interval of 30 minutes, and sol B is formed after being fully stirred; (3) The sol obtained in step (2) is put into a sealed 50ml plastic centrifuge tube and aged at 60° C. for 24 hours;

[0051] (4) The wet gel obtained in step (3) was washed 3 times with deionized water and absolute ethanol respectively;

[0052] (5) drying the wet gel in step (4) at 37° C. for 24 hours, and then freeze-drying for 24 ...

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Abstract

The invention discloses a biological active microsphere and a preparation method thereof. Based on molar percentage, the microsphere is composed of 16- 36% of CaO, 4% of P2O5 and 60-80% of SiO2 composition; the particle size of the microsphere 1-5 [mu]m; and the microsphere is in a hollow ball shape. The preparation method of the biological active microsphere comprises the following steps of preparing an acid catalyst polyethylene glycol solution A according to a proportion, adding tetraethoxysilane, triethyl phosphate and calcium nitrate terahydrate into the solution A respectively in order according to a molar ratio of 60-80 : 4 : 16-36 to form a gel B; hydrothermal ageing the gel B at the temperature of 60 DEG C and drying at the temperature of 37 DEG C to obtain a dry gel; grinding the dry gel and performing heat treatment on the grinded dry gel at the temperature of 600 DEG C-800 DEG C to obtain a target product. Compared with a conventional biological active microsphere, the biological active microsphere provided by the invention is in regular sphere shape with a hollow structure and smooth surface; the particle size of the microsphere 1-5 [mu]m; a particle size distribution range is narrow and uniform; and the biological active microsphere has excellent fluidity, syringeability and security and is simple in preparation process.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and in particular relates to a hollow bioactive microsphere and a preparation method thereof. Background technique [0002] Bioactive microspheres, such as bioactive glass microspheres, have been widely used in medical fields such as bone and tooth restoration and drug release carrier materials due to their good biological activity. Based on the anti-infection, minimum damage and adaptability requirements of the body for surgical and pharmaceutical excipients, the active microspheres are generally required to have good fluidity, extrudability and regular spherical shape. Especially in bone tissue defect filling surgery, the shape of irregular particles is likely to cause inflammation in the human body and affect the speed of bone formation. Therefore, it is generally preferred to use spherical particles with smooth surfaces as materials for tissue defect filling. Howe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/50A61L27/56C03C3/097B01J13/02
Inventor 林才李彬
Owner 佛山今兰生物科技有限公司
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