Animal viral vaccine pulsatile release system, and preparation method and application thereof

A technology of pulse release and release system, which can be used in antiviral agents, viral antigen components, pharmaceutical formulations, etc., and can solve the problem of low immunogenicity

Inactive Publication Date: 2013-08-21
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional inactivated vaccines, as well as genetically engineered subunit vaccines, synthetic peptide vaccines, and DNA vaccines that represent future development directions, all have shortcomings, that is, low immunogenicity, and multiple repeated vaccinations are required to produce effective immune protection.

Method used

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  • Animal viral vaccine pulsatile release system, and preparation method and application thereof
  • Animal viral vaccine pulsatile release system, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Preparation of Newcastle Disease Inactivated Vaccine Antigen

[0014] Take Newcastle disease attenuated La Sota strain, inoculate susceptible chicken embryos according to conventional methods, after culture, harvest chicken embryo liquid, and inactivate with formaldehyde solution. Take the inactivated Newcastle disease virus chicken embryo liquid, centrifuge at 4°C under sterile conditions to get the supernatant, and then ultrafilter the supernatant through an ultrafiltration membrane with a molecular weight cut-off of 30,000 under sterile conditions at 4°C concentrate. The supernatant after ultrafiltration purification and concentration is the antigen solution for preparing vaccine microspheres.

Embodiment 2

[0016] Preparation I of Newcastle Disease Inactivated Vaccine Microspheres

[0017] Preparation of microspheres: PLGA microspheres of Newcastle disease inactivated vaccine were prepared by drying in double emulsion. The Newcastle disease antigen solution was used as the water phase, and PLGA (50:50) was dissolved in dichloromethane to prepare a solution with an appropriate concentration as the oil phase. Mix the water phase and the oil phase at a ratio of 1:10 (V / V), and ultrasonically emulsify for 10 seconds in an ice bath to obtain colostrum. Add the colostrum into the 2% PVA solution, and stir it through an emulsifier at high speed for 30 seconds at 10,000 rpm to obtain double milk. Transfer the obtained double emulsion to a beaker, stir magnetically at 400rpm for 5 hours, evaporate dichloromethane at room temperature, and then centrifuge at 6000rpm for 10 minutes to collect the microspheres, wash with sterilized water 3 times, and vacuum freeze Dry to get Newcastle disea...

Embodiment 3

[0023] Preparation of Newcastle Disease Inactivated Vaccine Microspheres II

[0024] Preparation of microspheres: PLGA microspheres of Newcastle disease inactivated vaccine were prepared by drying in double emulsion. The Newcastle disease antigen solution was used as the water phase, and PLGA (75:25) was dissolved in dichloromethane to prepare a solution with an appropriate concentration as the oil phase. Mix the water phase and the oil phase at a ratio of 1:10 (V / V), and ultrasonically emulsify for 10 seconds in an ice bath to obtain colostrum. Add the colostrum into the 2% PVA solution, and stir it through an emulsifier at a high speed for 30 seconds at 4000 rpm to obtain double milk. Transfer the obtained double emulsion to a beaker, stir magnetically at 200rpm for 5 hours, evaporate dichloromethane at room temperature, and then centrifuge at 5000rpm for 10 minutes to collect microspheres, wash with sterilized water 3 times, and vacuum freeze Dry to obtain Newcastle disea...

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Abstract

The invention relates to the field of biomedical engineering, and specifically relates to an animal viral vaccine pulsatile release system, a preparation method thereof, and an application thereof in animal disease prevention. According to the invention, a vaccine pulsatile release system composed of polymer microspheres comprising vaccine antigens and with different release characteristics and compositions of the microspheres; injection medication is adopted; and full immunization is realized with single dose injection. Therefore, immunization process is simplified, immunization effect is improved, labor intensity is reduced, and immunization missing rate is reduced.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to an animal vaccine pulse release system, its preparation method and its application in the prevention and treatment of animal epidemics. Background technique [0002] Vaccination is the most effective and practical way to prevent, control and eradicate zoonotic diseases. However, traditional inactivated vaccines, as well as genetically engineered subunit vaccines, synthetic peptide vaccines, and DNA vaccines that represent future development directions, all have shortcomings, that is, low immunogenicity and repeated vaccinations are required to produce effective immune protection. There are two ways to solve this problem, one is to develop a new type of high-efficiency immune adjuvant; the other is to develop a controlled release system for injectable vaccine antigens, which can deliver antigens to specific tissues in a constant, sustained and predetermined manner . [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K9/16A61K47/34A61K47/36A61K47/42A61P31/12
Inventor 李军曾魁蒋碧蓉
Owner HAINAN UNIVERSITY
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