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Magnetic nanometer gene vector system as well as preparation method and application thereof

A gene carrier, magnetic nanotechnology, applied in gene therapy, the introduction of foreign genetic material using a vector, and other methods of inserting foreign genetic material, etc., can solve the problems affecting the safety of gene transfection effect, high cytotoxicity, etc. Gene transfection efficiency, the effect of reducing cytotoxicity, particle size and surface charge

Active Publication Date: 2013-08-21
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these positively charged magnetic nanoparticles are prone to produce aggregates of magnetic nanoparticles during the preparation process (Acta Biomater, 2011. 7(3): p. 1319-26.), which are more likely to form when combined with the electrostatic interaction of genes Gene complexes with larger particle size and higher surface positive charge are prone to high cytotoxicity, which affects the effect of gene transfection in vitro and the safety of in vivo application

Method used

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  • Magnetic nanometer gene vector system as well as preparation method and application thereof
  • Magnetic nanometer gene vector system as well as preparation method and application thereof
  • Magnetic nanometer gene vector system as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A magnetic nanometer gene carrier system is composed of negatively charged magnetic nanoparticles (MNP), a cationic carrier and a gene. The gene carrier system is prepared by the following method:

[0031] The gene was dissolved in sterile water, and the cationic carrier and negatively charged magnetic nanoparticles were dissolved in sterile buffer solution; according to the mass ratio of the cationic carrier to the gene was 0.3:1, the cationic carrier was added to the gene solution, at room temperature Leave it for 8 minutes to obtain the solution binary gene complex; then add negatively charged magnetic nanoparticles into the binary gene complex according to the ratio of iron element to gene mass ratio of 0.2:1 (the average of the magnetic nanoparticles The particle size is 20nm, and the surface potential is

[0032] -25mV), and placed at room temperature for 6 minutes to obtain a three-component magnetic nano-gene carrier system.

Embodiment 2

[0034] A magnetic nanometer gene carrier system is composed of negatively charged magnetic nanoparticles (MNP), a cationic carrier and a gene. The gene carrier system is prepared by the following method:

[0035] The gene was dissolved in sterile water, and the cationic carrier and negatively charged magnetic nanoparticles were dissolved in sterile buffer respectively; according to the mass ratio of the cationic carrier to the gene was 45:1, the gene solution was added to the cationic carrier, at room temperature Place it for 30 minutes to obtain the solution binary gene complex; then add negatively charged magnetic nanoparticles into the binary gene complex according to the ratio of the mass ratio of the iron element to the gene of 10:1 (the average of the magnetic nanoparticles The particle size is 50nm, and the surface potential is

[0036] -48mV), and placed at room temperature for 25 minutes to obtain a ternary magnetic nano-gene carrier system.

Embodiment 3

[0038] A magnetic nanometer gene carrier system is composed of negatively charged magnetic nanoparticles (MNP), a cationic carrier and a gene. The gene carrier system is prepared by the following method:

[0039]The gene was dissolved in sterile water, and the cationic carrier and negatively charged magnetic nanoparticles were dissolved in sterile buffer respectively (the average particle diameter of the magnetic nanoparticles was 12nm, and the surface potential was -18mV); The mass ratio of the iron element to the gene is 12:1, and the cationic carrier is added to the negatively charged magnetic nanoparticles, and left at room temperature for 18 minutes to obtain a binary complex; Add the gene solution into the binary complex, and place it at room temperature for 12 minutes to obtain a ternary magnetic nano-gene carrier system.

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Abstract

The invention discloses a magnetic nanometer gene vector system as well as a preparation method and an application thereof. The gene vector system comprises electronegative magnetic nanoparticles, positive-ion vectors and genes, wherein the three components are prepared into the magnetic nanometer gene vector system according to certain proportions and composite sequences through electrostatic interactions. The magnetic nanometer gene vector system disclosed by the invention is simple in preparation method and has physicochemical properties of small grain diameter and proper surface charge. Compared with non-magnetic nanometer composites, the magnetic nanometer gene vector system has the advantages that the transfection efficiency to cells of the system is remarkably improved under the action of an additional magnetic field; and meanwhile, the cytotoxicity of the system is further reduced.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a magnetic nano-gene carrier system, a preparation method and an application. Background technique [0002] In recent years, gene therapy has become one of the most active fields in the research of genetic diseases and tumor treatment. The study of gene carrier is the core of gene therapy research. Due to the advantages of safety and low toxicity of non-viral gene vectors, it has become a hotspot in the research of gene vectors. Most non-viral vectors show high gene transfection efficiency in vitro, but low transfection efficiency in target tissues in vivo. Therefore, in the process of gene therapy, the targeting of gene carrier is one of the bottlenecks of gene therapy. [0003] The magnetic targeting gene delivery system has the advantages of high gene expression efficiency in vitro and good targeting in vivo, making it a very promising vector system. At present, the mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87C12N15/85A61K48/00
Inventor 顾忠伟聂宇谢丽何一燕姜倩岳冬江雯刘克霞
Owner SICHUAN UNIV
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