Method for industrially purifying sheep placenta polypeptides

A sheep placenta, dispersion technology, applied in the direction of pharmaceutical formulations, peptide/protein components, medical preparations containing active ingredients, etc., can solve problems such as unsatisfactory society

Inactive Publication Date: 2013-08-28
LANZHOU DADE AGRI & ANIMAL HUSBANDRY SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, domestic research on the function and composition of placental peptide, the active ingredient of sheep placenta, has matured. Its preparation methods are mainly homogenate di

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  • Method for industrially purifying sheep placenta polypeptides
  • Method for industrially purifying sheep placenta polypeptides
  • Method for industrially purifying sheep placenta polypeptides

Examples

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Embodiment Construction

[0054] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The methods in the following examples are conventional methods unless otherwise specified. The materials or instruments used in the following examples are all commercially available unless otherwise specified.

[0055] 1. Prepare sheep placenta freeze-dried powder

[0056] Wash 400kg sheep placenta with industrial pure water, cut it into pieces (remove fascia, blood vessel and fat), mechanically crush it for pretreatment, then add 0.154 mol / L normal saline with a mass ratio of 1:4 to 280W Disperse for 15 minutes under ultrasonic action to form a homogenate. After homogenization, freeze for three consecutive times under vacuum conditions. The first freeze is at -15°C for 3 hours, then the temperature is adjusted to -25°C for 8 hours, and finally the temperature is reduced to -30°C for 5 hours. After being dissolved and melted at 28°C, it is filtered th...

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Abstract

The invention provides a method for industrially purifying sheep placenta polypeptides. The method comprises the following steps: preprocessing and homogenating sheep placentas, continuously carrying out three-time refrigeration under a vacuum condition at different temperatures, filtering, refrigerating, drying, and crushing to prepare lyophilized sheep placenta powder; carrying out a supersonic wave and microwave heating compounding process of the lyophilized sheep placenta powder for dispersion to form a tissue homogenate; cooling the tissue homogenate, centrifuging, and taking the obtained supernatant; adding a mixed organic solvent into the supernatant, fully reacting, centrifuging, taking the obtained new supernatant, adding acetone to the new supernatant for extraction, and taking the obtained precipitate; and dissolving the precipitate, letting the obtained precipitate solution in an ultrafiltration membrane assembly separator and a gel column combined separator for combined purification, and carrying out concentration lyophilizing to obtain purified sheep placenta polypeptides. The method has a low production cost and is suitable for the industrialized production, and a detection result shows that the purity of the sheep placenta polypeptides prepared through the method reaches above 90%.

Description

Technical field [0001] The invention relates to a purification method, in particular to a method for industrially purifying sheep placental polypeptides. Background technique [0002] Sheep placenta polypeptide is a small molecule peptide with biological activity extracted from sheep placenta. Sheep placenta is rich in all the primitive life substances necessary for life. Modern medical research has found that sheep placenta is rich in protein, 17 kinds of amino acids, 14 kinds of trace elements, phospholipids, lipopolysaccharides, vitamins, and a variety of active peptides related to immune function and normal functioning of the body, which can regulate the body’s immunity , Improve microcirculation, reduce blood cholesterol, regulate the body secretion and other functions. Scientists have confirmed through a large number of experiments that sheep placenta also has the effects of nourishing blood, soothing the nerves, enriching skin and skin, prolonging life, and its natural r...

Claims

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Application Information

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IPC IPC(8): A61K35/50A61K38/02
Inventor 陈伟
Owner LANZHOU DADE AGRI & ANIMAL HUSBANDRY SCI & TECH
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