Method for industrially purifying sheep placenta polypeptides
A sheep placenta, dispersion technology, applied in the direction of pharmaceutical formulations, peptide/protein components, medical preparations containing active ingredients, etc., can solve problems such as unsatisfactory society
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[0054] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The methods in the following examples are conventional methods unless otherwise specified. The materials or instruments used in the following examples are all commercially available unless otherwise specified.
[0055] 1. Prepare sheep placenta freeze-dried powder
[0056] Wash 400kg sheep placenta with industrial pure water, cut it into pieces (remove fascia, blood vessel and fat), mechanically crush it for pretreatment, then add 0.154 mol / L normal saline with a mass ratio of 1:4 to 280W Disperse for 15 minutes under ultrasonic action to form a homogenate. After homogenization, freeze for three consecutive times under vacuum conditions. The first freeze is at -15°C for 3 hours, then the temperature is adjusted to -25°C for 8 hours, and finally the temperature is reduced to -30°C for 5 hours. After being dissolved and melted at 28°C, it is filtered th...
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