Method for producing hydrolyzed wheat protein

A technology of wheat hydrolyzed protein and production method, which is applied in the field of comprehensive development and application of wheat deep processing, which can solve the problems of high price of wheat hydrolyzed protein, difficulty in controlling the enzymatic hydrolysis process, difficult separation of target products, etc., and achieve low free amino acid content and high degree of hydrolysis Appropriate, soft-tasting effect

Active Publication Date: 2013-09-04
江苏联海生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the enzymatic hydrolysis process is easy to produce by-products, the enzymatic hydrolysis process is difficult to control, resulting in incomplete hydrolysis, poor taste of hydrolyzed products, and difficult separation of target products
At the same time, the price of enzyme pr

Method used

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  • Method for producing hydrolyzed wheat protein
  • Method for producing hydrolyzed wheat protein

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Embodiment 1

[0019] A kind of production method of wheat hydrolyzed protein of the present invention, comprises the following steps:

[0020] a, preparation of enzymolysis solution: add 3000g of water into the reaction kettle, heat to 50°C, add 400g of gluten powder to the reaction kettle at a ratio of 8 U / g gluten powder, and add compound protease at 100r Stir at a speed of 1 / min, the stirring time is 2 minutes, then add 300g gluten powder, stir at a speed of 250r / min, the stirring time is 6 minutes, then add 300g gluten powder, stir at a speed of 400r / min, add alkali to adjust the pH Value, the range of pH value is 7.0-7.5, after reacting for 20 minutes, add medium temperature amylase according to the amount of 2U / g gluten powder, stir at 300r / min speed, after reacting for 10 minutes, according to the amount of 30U / g gluten powder Add glucoamylase in an appropriate amount, stir at 200r / min, and react for 5 minutes. After hydrolysis, raise the temperature of the enzymolysis solution to 75...

Embodiment 2

[0026] A kind of production method of wheat hydrolyzed protein of the present invention, comprises the following steps:

[0027] a, the preparation of enzymolysis solution: the water of 3000g is added in the reactor, is heated to 50-65 ℃, with the gluten powder of 300g, adds compound protease in the ratio of 15 U / g gluten powder in the reactor, Stir at a speed of 400r / min for 5 minutes, then add 400g gluten powder, stir at a speed of 250r / min, stir for 2 minutes, then add 300g of gluten, stir at a speed of 100r / min, add alkali Adjust the pH value, the range of pH value is 7.0-7.5, after reacting for 40 minutes, add medium temperature amylase according to the amount of 3U / g gluten powder, stir at 300r / min, after reacting for 15 minutes, according to the amount of 40U / g gluten Add glucoamylase to the amount of powder, and keep stirring at a stirring speed of 200r / min. React for 10 minutes. After hydrolysis, heat the enzymolysis solution to 85°C and keep it for 6 minutes. Cool t...

Embodiment 3

[0033] A kind of production method of wheat hydrolyzed protein of the present invention, comprises the following steps:

[0034] a. Preparation of enzymolysis solution: add 3000g of water into the reactor, heat to 65°C, add 300g of gluten powder to the reactor at a ratio of 20 U / g gluten powder, and add compound protease at 400r Stir at a speed of 1 / min, the stirring time is 8 minutes, then add 300g gluten powder, stir at a speed of 300r / min, the stirring time is 6 minutes, then add 400g gluten powder, stir at a speed of 100r / min, add alkali to adjust the pH value, the range of pH value is 7.0-7.5, after reacting for 60 minutes, add medium temperature amylase according to the amount of 5U / g gluten powder, stir at 400r / min speed, after reacting for 20 minutes, according to the amount of 50U / g gluten powder Add glucoamylase in an appropriate amount, and keep stirring, the stirring speed is 400r / min, and react for 15 minutes. Liquid cooling to 35°C;

[0035] B, the preparation ...

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Abstract

The invention discloses a method for producing hydrolyzed wheat proteins. The method is characterized by comprising the following steps of: preparing enzymatic hydrolysate, namely adding 1,000 parts of vital gluten in mass for three times according to the weight ratio of the vital gluten to water being 1 to 5-3, after hydrolysis is performed, raising the temperature of the enzymatic hydrolysate to 75-100 DEG C, keeping the temperature of the enzymatic hydrolysate for 3-10 minutes, performing enzyme deactivation on the enzymatic hydrolysate, cooling the enzymatic hydrolysate subjected to enzyme deactivation to 30-35 DEG C, after fermentation is finished, filtering fermented liquid, and finally drying the filtrate by spraying. The method has the advantages that by a multi-enzymolysis and microorganism fermentation combination method, a novel hydrolyzed wheat protein product is developed by fully combining characteristics of a protein structure of wheat gluten proteins, the cleavage sites of protease, biological conversion of microorganisms, animal nutrition absorption and the like, so that the taste and the flavor of the product are greatly improved; the utilization of enzyme preparations can be greatly reduced; the production cost is reduced; the production technology is simple and novel; the period is short; three industrial wastes are not produced; and the hydrolyzed wheat proteins are environmentally-friendly.

Description

technical field [0001] The invention relates to a production method of wheat hydrolyzed protein, in particular to a process for industrialized production of wheat hydrolyzed protein, a method for processing protein additives for feed, and belongs to the field of comprehensive development and application of deep processing of wheat. Background technique [0002] Gluten powder, also known as wheat gluten, is a by-product of the industrial production of wheat starch. It is a powdery product mainly made by washing, separating and drying. It is a cheap, high-quality, pure natural plant protein. Its protein content is as high as 72%~85%, containing 15 kinds of amino acids necessary for the human body, of which the glutamine content is as high as 30%, and the proline content is about 15%. It is rich in nutrients and has important physiologically active functions. The solubility is very poor, and when it is used as a feed protein source, its biological value and efficacy ratio are v...

Claims

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Application Information

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IPC IPC(8): A23J3/18A23J3/34
Inventor 唐波仝志勇陈玉和
Owner 江苏联海生物科技有限公司
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