Unlock instant, AI-driven research and patent intelligence for your innovation.

Silk fibroin nanocrystalline and preparation method thereof

A technology of silk fibroin and nanocrystals, applied in the fields of protein, polymer chemistry, and nanomaterials

Inactive Publication Date: 2013-09-04
NANJING UNIV OF INFORMATION SCI & TECH
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent uses organic solvents as denaturants, which may reduce the biocompatibility of silk fibroin, which has limitations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Silk fibroin nanocrystalline and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Pretreatment: Add 400 ml of deionized water to 10 grams of silk raw material, and add 2 grams of anhydrous sodium carbonate, put it in a water bath at a temperature of 100 ° C, boil for 90 minutes, and then clean it with distilled water;

[0034] Add the cooked silk into distilled water (the concentration of silk is 15% by weight), soak for 12 hours, pour it into a ball mill and grind it for 5 times, each time for 10 minutes, put the obtained raw material into an oven at 37°C to dry Let dry for 24 hours.

Embodiment 2

[0036] Chemical hydrolysis: the degummed silk sample of 1 gram embodiment 1 is added 50 milliliters of phosphoric acid solutions (mass concentration is 75%), 35 degrees of heating temperature, hydrolyze 1 hour, add 50 milliliters of distilled waters and dilute, then the hydrolyzed solution is transferred to molecular weight cut-off: 12000 dialysis bags, dialyzed for 72 hours until the solution pH=7. The dialyzed solution was transferred to a centrifuge tube, and centrifuged once at a speed of 12000 rpm for 10 minutes to obtain a silk fibroin nanocrystal suspension.

Embodiment 3

[0038] Chemical hydrolysis: the degummed silk sample of 2 grams of example 1 is added 30 milliliters of phosphoric acid solutions (mass concentration is 75%), heating temperature is 30 degrees, hydrolyzes 1 hour, adds 60 milliliters of distilled waters and dilutes, then the hydrolyzed solution is transferred to molecular weight cut off and is 12000 In a dialysis bag, dialyze until the solution pH=7; transfer the dialyzed solution to a centrifuge tube, and centrifuge 5 times at a centrifuge speed of 12000 rpm for 8 minutes each time to obtain a silk fibroin nanocrystal suspension.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides silk fibroin nanocrystalline and a preparation method thereof. The method comprises the following steps: 1, preprocessing; 2, performing chemical hydrolysis; and 3, mechanically processing. By the method, preprocessed silk raw materials are hydrolyzed by inorganic acid to obtain silk fibroin nanocrystalline suspension which is well dispersed and has a uniform size; and the diameter of obtained nanoparticles is 100 nm plus and minus 50 nm. The method is low in cost of utilized resources, has a simple process in the synthetic process and is green and free from pollution; and the obtained product is green and environmental-friendly.

Description

technical field [0001] The invention relates to silk fibroin nanocrystals and a preparation method thereof, and belongs to the fields of polymer chemistry, nanometer materials and proteins. Background technique [0002] As a natural fibrous protein, silk is one of the earliest natural proteins used by humans, and has been favored by people since ancient times. It is a protein extracted from silk. It has good biocompatibility and can be prepared into various forms of materials such as membranes and gels. Due to its unique physical and chemical properties and good mechanical properties, it is low in cost and source Extensive and highly recyclable, silk fibroin materials are currently widely used in food, medicine, biomaterials and other fields. Silk fibroin nanocrystals have become a hotspot in the field of polymer research and have broad application prospects. [0003] In the Chinese invention patent (CN243059C), a preparation method of silk fibroin nanoparticles is disclos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K47/42
Inventor 刘大刚陈鹏
Owner NANJING UNIV OF INFORMATION SCI & TECH