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Method for extracting volatile oil from agilawood seeds, and usage and application of volatile oil

A carbon dioxide and volatile oil technology, which is applied in the fields of health care and biomedicine, can solve problems such as complex processes, ecological imbalance of deep-sea sharks, and difficult control of operating processes, and achieve the effects of simple process flow, preventing the spread of cancer cells, and inhibiting the formation of cancer cells

Inactive Publication Date: 2015-04-15
ZHONGSHAN TRADITIONAL CHINESE MEDICINE HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high content of squalene in the liver of deep-sea sharks, such as 40%-74% in armored shark liver oil, and 49%-89% in small spiny sharks, most of them currently use deep-sea shark liver oil to extract squalene; but The production of squalene in such a large amount will lead to the ecological imbalance of deep-sea sharks
[0005] In recent years, a small amount of methods for extracting squalene from plants have also been announced. Chinese patent (CN201110027119.4) "Method for extracting squalene from camellia oleifera seeds and tea oil squalene soft capsules" is based on extraction, chromatographic separation Combined with normal temperature extraction and supercritical extraction several extraction methods to extract suitable high-purity squalene; The separation process combined with silica gel chromatography extracts high-purity squalene; the Chinese patent (CN201010190275.8) "Method for Extracting Squalene from Vegetable Oil Deodorization Distillate" uses molecular distillation, extraction, and crystallization to extract high-purity squalene. Concentration of squalene; these methods require a combination of multiple extraction methods, the process is more complicated, and other chemical substances are introduced, and it is difficult to control the process

Method used

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  • Method for extracting volatile oil from agilawood seeds, and usage and application of volatile oil
  • Method for extracting volatile oil from agilawood seeds, and usage and application of volatile oil
  • Method for extracting volatile oil from agilawood seeds, and usage and application of volatile oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Take the dried agarwood seeds with a water content of ≤10%, crush them with a pulverizer to pass through a 40-mesh sieve, weigh 300g accurately and place them in an extraction kettle with 20L / h of CO 2 Extract at 40°C and 25MPa for 1.5h, then collect the yellow transparent oil with strong aroma in the first separation kettle (pressure 7MPa, temperature 50°C) and the second separation kettle (pressure 6MPa, temperature 50°C) under reduced pressure , namely 132g of volatile oil, the extraction rate is 44%, and the extraction rate of squalene is 16.72%.

Embodiment 2

[0064] Take the dried agarwood seeds with a water content of ≤10%, crush them with a pulverizer until they pass through a 30-mesh sieve, weigh 300g accurately and place them in an extraction kettle with 20L / h of CO 2 Extract at 40°C and 18MPa for 2h, and then collect the yellow transparent oil with strong fragrance under reduced pressure in the first separation kettle (pressure 6MPa, temperature 48°C) and the second separation kettle (pressure 5MPa, temperature 48°C). That is 117g of volatile oil, the extraction rate is 39%, and the extraction rate of squalene is 14.04%.

Embodiment 3

[0066] Take the dried Agarwood seeds with a water content of ≤10%, crush them with a pulverizer to pass through a 20-mesh sieve, weigh 300g accurately and place them in an extraction kettle with 20L / h of CO 2 Extracted at 40°C and 21MPa for 2.5h, then collected the yellow transparent oil with strong aroma in the first separation kettle (pressure 7MPa, temperature 45°C) and the second separation kettle (pressure 5MPa, temperature 45°C) under reduced pressure , namely 147g of volatile oil, the extraction rate is 49%, and the extraction rate of squalene is 19.11%.

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Abstract

The invention discloses a method for extracting a volatile oil from agilawood seeds, and a usage and an application of the volatile oil. In the method disclosed by the invention, a supercritical CO2 fluid extraction method is adopted to extract volatile components from aquilaria sinensis seeds; and a DPPH method is used to study anti-oxidation capability of effective components of the volatile components. With the method, squalene with relatively high content is detected in the aquilaria sinensis seeds for the first time; and the volatile oil has a certain anti-oxidation capability, so that scientific basis is provided for further studying active components of the aquilaria sinensis seeds development and utilization The extraction method is simple in process flow, high in extraction yield and low in extraction temperature, and can prevent degradation of heat-sensitive components. The method fully develops rich resources of the agilawood rare medicinal material, and makes the best use of the agilawood. With the method, effective components in the agilawood seeds are extracted simply and effectively, thereby developing novel raw materials and extraction process for the squalene.

Description

【Technical field】 [0001] The invention relates to a method for extracting the extract of agarwood seeds, in particular to a method for extracting volatile oil from the seeds of agarwood. The invention also relates to the use and application of the volatile oil of the agarwood seed extract, belonging to the technical fields of biomedicine and health care. 【Background technique】 [0002] Aquilaria sinensis (lour.) Gilgl. Resin-containing wood is called agarwood, also known as domestic agarwood, earth agarwood or Guanxiang. Aquilaria sinensis is also the only plant resource for producing agarwood medicinal materials in my country. The wild resources of Acacia sinensis are on the verge of extinction, and it has been listed as a national rare and endangered third-level protected plant and a national second-level key protected plant. Agarwood, pungent, bitter, warm in nature, has the effects of promoting qi and relieving pain, warming the middle and relieving vomiting, inducing qi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/835A61K9/48A61K8/11A61K8/97A23L1/29A61P35/00A61P39/00A61P9/00A61P31/00A61P39/06A61Q19/00A61Q19/08A61K131/00A23L33/00
Inventor 吴惠妃李庆国梅全喜
Owner ZHONGSHAN TRADITIONAL CHINESE MEDICINE HOSPITAL
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