Method for preparing sub totipotential stem cell by utilizing placenta lobular tissue

Abstract

The invention discloses a method for preparing a sub totipotential stem cell by a utilizing placenta lobular tissue. The method is characterized by comprising the following steps of: preparing cell suspension by utilizing the placenta lobular tissue after pretreating placenta, and preparing sub totipotential stem cell separating medium by utilizing the cell suspension; and carrying out primary culture and a second-generation culture on the sub totipotential stem cell separating medium, and collecting fully cast-off cells in the second-generation culture process to obtain the sub totipotential stem cell; and cooling and freezing in a programmed manner, taking out the frozen sample after cooling the temperature to -80 DEG C to -90.0 DEG C, and placing the frozen sample in a liquid-nitrogen storage tank for storing for a long time. The method for preparing the sub totipotential stem cell by utilizing the placenta lobular tissue has the advantages that the passage capacity can reach 20 generations, so that not only the capacity of differentiating towards bones, cartilages, fat and nerve cells can be achieved, but also the capacity of differentiating towards islet cells and epidermis cells be achieved; and the sub totipotential stem cell is uniform, stable, short in production period, simple to operate and low in cost.

Description

technical field [0001] The invention relates to the field of stem cell preparation, in particular to a method for preparing subtotipotent stem cells by using placenta tissue. Background technique [0002] Stem cell technology is one of the hottest technologies in life science today. Its research content involves almost all biomedical fields of life science. In addition to playing an important role in cell therapy, tissue / organ transplantation and gene therapy, it is also discovering new genes , Gene function analysis, developmental biology models and new drug development have an important impact. Sub-totipotent stem cells (sub-totipotent stem cells) are stem cells derived from mature placenta tissue, which have high self-renewal ability and multi-lineage differentiation potential. They are new seed cells for cell transplantation and tissue engineering, and have broad application prospects. [0003] Subtotipotent stem cells are an emerging and more promising project in the s...

AI Technical Summary

Problems solved by technology

The Chinese invention patent name is sub-totipotent stem cells, its preparation method and its use (application number CN200810240040.8) discloses a sub-totipotent stem cell, which is derived from a cut human umbilical cord or placenta, and the cell marker is CD151+OCT4+CD184-, It grows adherently in a culture vessel and can differentiate into human body, mesoderm and ectoderm tissues. Its preparation process needs to be expanded to more than four generations to obtain enough cells for the establishment of a sub-totipotent stem cell seed bank, and its proliferation ability is weak , a slower growth rate