Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of barley straws and tea seed cakes
A technology of probiotic preparation and barley straw, which is applied in animal feed, animal feed, application, etc., can solve the problems of difficult digestion of monogastric animals, and achieve the effects of low-cost and high-efficiency biotransformation, no waste water discharge, and easy operation
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Embodiment 1
[0027] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of barley straw, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, at 35°C After 48 hours of acclimatization and cultivation, a leavening agent is formed;
[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of barley straw, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.
[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 55.25IU / g; total cellulase activity reaches 6.12IU / g; beta-glucosidase activity reaches 55.25IU / g; reached 12.43IU / g; endoglucanase activity reached 43.19IU / ...
Embodiment 2
[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of barley straw, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;
[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of barley straw, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.
[0033] After measurement, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 54.98IU / g; total cellulase activity reaches 6.08IU / g; beta-glucosidase activity reaches 54.98IU / g; reached 12.36IU / g; endoglucanase activity reached 43.04IU / g; xylanas...
Embodiment 3
[0035] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of barley straw, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, at 40°C After 36 hours of acclimatization and cultivation, a leavening agent is formed;
[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of barley straw, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.
[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 54.60IU / g; total cellulase activity reaches 6.32IU / g; beta-glucosidase activity reaches 54.60IU / g; reached 13.12IU / g; endoglucanase activity reach...
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