Method for separating soluble grape seed protein by use of membrane and cleaning method of membrane

A grape seed, soluble technology, applied in semi-permeable membrane separation, peptide preparation methods, chemical instruments and methods, etc. problem, to achieve the effect of easy recovery, excellent membrane flux, increased speed and

Inactive Publication Date: 2013-09-11
甘肃凯源生物技术开发中心有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN1680359A discloses a method for extracting oligomeric proanthocyanidins from grape seeds by ultrafiltration with a modified polyethersulfone flat membrane, and the obtained oligomeric proanthocyanidins are as high as 95%, but this method is not suitable for the separation of grape seed proteins Extraction, there is no report on the separation of soluble grape seed protein by membrane separation technology in the existing literature
And it is well known in the art that because grape seeds contain a large amount of substances remaining on the membrane, it is difficult to be removed, so membrane technology has not been applied in the separation of soluble glucose proteins.

Method used

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  • Method for separating soluble grape seed protein by use of membrane and cleaning method of membrane
  • Method for separating soluble grape seed protein by use of membrane and cleaning method of membrane
  • Method for separating soluble grape seed protein by use of membrane and cleaning method of membrane

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Effect test

Embodiment 1

[0045] A method for membrane separation of soluble grape seed protein, the method steps are as follows:

[0046] Grape pomace mixture in wine fermentation leftovers, dried in the sun, selected clean grape seeds (containing 12% protein);

[0047] Add petroleum ether to the grape seed powder obtained after removing impurities, washing with water, drying, and pulverizing the selected grape seeds, the ratio of solid to liquid of the grape seed powder and petroleum ether is 1:9, soak at room temperature for 9 hours, filter with gauze, Dried to obtain defatted grape seeds;

[0048]Then add the defatted grape seeds to the pH buffer solution, the solid-liquid ratio of the defatted grape seeds to the pH buffer solution is 1:100, the pH of the resulting mixed solution is controlled at 6, and the mixed solution is subjected to ultrasonic-microwave combined treatment 5min, and remove impurities through 8 layers of gauze suction filtration to obtain grape seed protein extract;

[0049] U...

Embodiment 2

[0054] A method for membrane separation of soluble grape seed protein, the method steps are as follows:

[0055] The grape seed powder obtained after removing impurities, washing with water, drying, and pulverizing the grape seeds was added to petroleum ether, and the solid-liquid ratio of the grape seed powder to petroleum ether was 1:10, then soaked at room temperature for 10 hours, and filtered through gauze. Dried to obtain defatted grape seeds;

[0056] Then add the defatted grape seeds to the pH buffer solution, the solid-liquid ratio of the defatted grape seeds to the pH buffer solution is 1:120, the pH of the resulting mixed solution is controlled at 9, and the mixed solution is subjected to ultrasonic-microwave combined treatment 10min, and remove impurities by suction filtration through 8 layers of gauze to obtain grape seed protein extract;

[0057] Under the applied pressure of 0.125MPa, the outer diameter is 30mm, the length is 500mm, the membrane pore diameter i...

Embodiment 3

[0061] A method for membrane separation of soluble grape seed protein, the method steps are as follows:

[0062] Add petroleum ether to the grape seed powder obtained after removing impurities, washing with water, drying, and pulverizing the grape seeds. The ratio of solid to liquid between the grape seed powder and petroleum ether is 1:11, soak at room temperature for 11 hours, and filter with gauze. Dried to obtain defatted grape seeds;

[0063] Then add the defatted grape seeds to the pH buffer solution, the solid-liquid ratio of the defatted grape seeds to the pH buffer solution is 1:150, the pH of the resulting mixed solution is controlled at 8, and the mixed solution is subjected to ultrasonic-microwave combined treatment 20min, and remove impurities through 8 layers of gauze suction filtration to obtain grape seed protein extract;

[0064] Under the external pressure of 0.150MPa, the outer diameter is 30mm, the length is 500mm, the membrane pore diameter is 50nm, and t...

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Abstract

The invention discloses a method for separating soluble grape seed protein by use of a membrane, which comprises the following steps of: preparing grape seeds into degreased grape seeds; adding a pH buffer solution with pH of 5-10 into the degreased grape seeds according to a feed liquid ratio of (1:100)-(1:150); performing ultrasonic-microwave combined treatment for 5-20 minutes; performing suction filtration to obtain soluble grape seed protein extraction liquid with viscosity of 30-90mPa.s, wherein the feed liquid ratio is the ratio of gram weight to the milliliter volume; performing circular treatment on the grape seed protein extraction liquid under a pressure of 0.025-0.150MPa by use of an inorganic separation membrane; and collecting the intersected solution of soluble grape seed protein. The method disclosed by the invention has the advantages of low energy consumption, no phase change of substances in a separation process, good separation effect, simplicity in operation, easiness in recovery of separation products and the like; and the speed of the separation process and the comprehensive utilization rate of grape seeds are improved, and the recovery rate of grape seed protein exceeds 89.45%.

Description

technical field [0001] The invention belongs to the field of separation and extraction methods for active ingredients of natural products, in particular to a method for membrane separation of soluble grape seed protein. technical background [0002] The Hexi Corridor is located between 36° and 40° north latitude. It has the best light, heat, water, soil and other resources for producing high-quality wine grapes. It is one of the best organic wine producing areas in China and even in the world. In the wine processing process, a large amount of pomace waste, which accounts for 20-30% of the grape processing volume, is produced every year, mainly including grape skins, grape seeds and fruit stems. The research on grape seeds mainly focuses on grape seed oil, polyphenols, and alcohols, and the development and utilization of protein in grape seeds is less. Grape seed protein contains 17 kinds of amino acids, including 8 kinds of amino acids necessary for human body. Therefore, e...

Claims

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Application Information

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IPC IPC(8): C07K1/34B01D65/06
Inventor 张喜峰罗光宏祖廷勋张芬琴
Owner 甘肃凯源生物技术开发中心有限责任公司
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