Bacillus vallismortis strain SJ and application thereof in preparation of tobacco antiviral preparation and promoter
A technology of bacillus and bacterial preparations in the field of flowers, applied in the field of microorganisms, can solve problems that affect the sustainable development of tobacco leaf production, reduce the effect of chemical agents on disease prevention, and reduce the internal quality of tobacco leaves, etc., achieve good technical effects and market application prospects, and facilitate expansion The effect of efficient production and long storage time
Active Publication Date: 2013-09-11
青岛海大生物集团股份有限公司
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Problems solved by technology
At present, the general method of controlling tobacco diseases is to use chemical pesticides. However, long-term use of chemical pesticides will lead to resistance of pathogenic bacteria and reduce the disease prevention effect of chemical agents. At the same time, long-term use of chemical agents will cause excessive pesticide residues and environmental pollution. Intrinsic quality declines, the ecological imbalance of the tobacco field, and even seriously affects the sustainable development of tobacco leaf production
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Embodiment 1
[0019] 1. Isolation and purification of active strains
[0020] On July 4, 2012, a total of more than 50 samples of rhizosphere soil were sampled from tobacco planting soil in Yishui Tobacco Area, Shandong, and 2g samples were suspended in 20ml of normal saline, 180rpm, and shaken for 30min. Take 100 μL of the sample suspension and evenly spread it on the nutrient agar plate, incubate it upside down in a 35°C incubator for 16 hours, pick single colonies with different shapes and inoculate them on the nutrient agar plate by streaking, and obtain pure A total of 126 pure bacteria were screened out, and all the strains were preserved on the slope.
[0021] Two, the screening of the SJ bacterial strain of preventing and treating TMV antagonistic bacteria
[0022] Pick the above-mentioned purified strains and inoculate them in LB liquid medium, keep the temperature at 28°C, shake and culture at 140rpm for 48 hours, take 20ml and mix it with an equal volume of 20 times TMV juice fo...
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Abstract
The invention discloses a bacillus vallismortis strain SJ and an application of the strain in preparation of a tobacco antiviral preparation and a promoter. The preservation number of the bacillus vallismortis strain SJ is CGMCC No.7571 (China General Microbiological Culture Collection Center). The invention also provides an SJ strain preparation with certain viable count, and the SJ strain preparation can be used for preparing a biological preparation capable of resisting a tobacco viral disease and a promoter for promoting the growth of the tobacco. By adopting the technical scheme, not only the utilization rates of a fertilizer and a pesticide capable of resisting the tobacco virus can be reduced, but also the environment pollution caused by the pesticide is reduced; the application of the SJ strain preparation disclosed by the invention not only can reach good antiviral effect, but also can effectively promote the growth of the tobacco; and the SJ strain preparation is low in production cost, simple and convenient to operate, long in storage time, easy to store and transport, conductive to factory expanded production, capable of achieving industrialization, and good in technical effect and market application prospect.
Description
technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Bacillus floridae strain SJ and its application in preparing tobacco antiviral preparations and growth-promoting agents. Background technique [0002] Tobacco is the main economic crop in my country, and its output and quality are related to the national economy and people's livelihood. At present, the general method of controlling tobacco diseases is to use chemical pesticides. However, long-term use of chemical pesticides will lead to resistance of pathogenic bacteria and reduce the disease prevention effect of chemical agents. At the same time, long-term use of chemical agents will cause excessive pesticide residues and environmental pollution. Intrinsic quality declines, and the ecological imbalance of tobacco fields even seriously affects the sustainable development of tobacco leaf production. Biological control is a new way to control plant diseases. It main...
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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P21/00C12R1/07
Inventor 翟熙伦单俊伟宋海妹乔来艳张术聪
Owner 青岛海大生物集团股份有限公司
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