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Method for preparing borneol by using microorganism separated from natural world

A natural, isolated technology, applied in microorganism-based methods, biochemical equipment and methods, and treatment of microorganisms with electricity/wave energy, etc., can solve problems such as low yield, achieve excellent overall cost, substrate tolerance and weather resistance and improved stereoselectivity

Inactive Publication Date: 2013-09-11
ANHUI JIMIN MEDICAL TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, it can be found from existing literature reports that borneol is often only used as a by-product in the transformation product, so the yield is relatively low

Method used

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  • Method for preparing borneol by using microorganism separated from natural world
  • Method for preparing borneol by using microorganism separated from natural world

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Embodiment Construction

[0033] The method for preparing borneol by using enzymes isolated from nature in the present invention includes in sequence: the screening and identification of strains, the induced variation of strains, the preparation of borneol, and the like. The present invention collects soil, pine bark, pine leaves, etc. from an environment rich in turpentine, and screens microbial strains by establishing an appropriate screening model, and screens some microorganisms that can use pinene as a substrate for growth and can transform pinene from nature. Produce borneol microbial strains and explore new methods for preparing borneol. Biotransformation includes enzymatic transformation and microbial transformation, and the essence of microbial transformation is actually an enzyme-catalyzed transformation through the enzyme system in microorganisms. The content of the present invention will be described below in conjunction with specific embodiments.

[0034] Step 1: Screening and Identifica...

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Abstract

The present invention relates to a method for preparing borneol by using a microorganism separated from natural world. The method sequentially comprises: bacterial screening and identification, bacteria induction mutation, and borneol preparation. According to the present invention, a microorganism klebsiella planticola acting on pinene is successfully separated, and plasma induction and chemical induction are performed, wherein solid culture is performed to obtain 5*10<8> cfu / g koji material, such that substrate tolerance, weathering resistance and stereoselectivity are increased, substrate tolerance concentration achieves 10%, and bornyl ester content in the produced bornyl acetate is 66-75%; and according to the method, the pinene microorganism method is adopted to produce the bornyl acetate, water vapor distillation is adopted to remove the unreacted pinene, and saponification hydrolysis is performed to prepare the borneol, such that the borneol produced by the method is close to nature borneol, the borneol content is 66-75%, quality of the borneol is higher than quality of the process product of the existing chemical method, and comprehensive cost of the method of the present invention is superior to comprehensive cost of the existing process.

Description

[0001] technical field [0002] The invention relates to a method for preparing borneol, in particular to a method for preparing borneol by using a microorganism isolated from nature that can catalyze the transformation of pinene into borneol. Background technique [0003] my country is a country with large resources of terpene essential oils such as turpentine oil and eucalyptus oil. In recent years, my country has canceled the export tax rebate for natural essential oils such as turpentine. The implementation of this policy has effectively promoted the industrialization of high value-added products such as natural essential oil chiral compounds. However, sulfuric acid and boric anhydride are often used as catalysts in production, which are flammable and explosive; oxalic acid and acetic anhydride are used as esterification agents, and the pollution in the production process is serious. Therefore, many researchers at home and abroad hope to isolate microorganisms that can...

Claims

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Application Information

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IPC IPC(8): C12P7/02C12N1/20C12N13/00C12N15/01C12R1/22
Inventor 卢文清
Owner ANHUI JIMIN MEDICAL TECH
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