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Method for amplification-free nucleic acid detection on optofluidic chips

A fluidic and nucleic acid technology, applied in the field of integrated optics, can solve problems such as the reaction environment of expensive reagents

Inactive Publication Date: 2013-09-11
RGT UNIV OF CALIFORNIA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

RT-PCR requires highly skilled operators, expensive reagents, and a strictly controlled reaction environment

Method used

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  • Method for amplification-free nucleic acid detection on optofluidic chips
  • Method for amplification-free nucleic acid detection on optofluidic chips
  • Method for amplification-free nucleic acid detection on optofluidic chips

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Embodiment Construction

[0014] Optofluidics is a rapidly developing field that involves processing the interaction of light and fluids (usually liquids) on a microscopic scale. At present, the main research trends include optical devices defined by fluids, optical particle manipulation in liquids, and optical particle detection and analysis, especially in the fields of biology and biomedicine.

[0015] We have invented a liquid core waveguide based optical flow control method for amplification-free nucleic acid detection, which maximizes the interaction between light and sample analytes. Based on the generation of hollow-core anti-resonance reflective optical waveguides (ARROW), we have developed an independent planar optical flow control platform with extremely high sensitivity for optical particle detection but does not require advanced microscope equipment. The basic layout of this platform and the manufacturing steps used to form the hollow core waveguide are in figure 1 Shown in.

[0016] in figure ...

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Abstract

An optofluidic platform is constructed so as to comprise a planar, liquid-core integrated optical waveguides for specific detection of nucleic acids. Most preferably, the optical waveguides comprises antiresonant reflecting optical waveguide (ARROWs). A liquid solution can be prepared and introduced into the optofluidic platform to for optical excitation. The resulting optical signal can be collected at the edges of the optofluidic platform and can be analyzed to determine the existence of a single and / or a specific nucleic acid.

Description

[0001] Cross references to related applications [0002] According to 35U.SC§119(e), this application requires the rights and interests of the U.S. Provisional Application No. 61 / 415,482 filed on November 19, 2010 under the title of "Method for Amplification-Free Nucleic Acid Detection on Optofluidic Chips" (Agency Case No. UCSC-0015 (SC2011-262)), the entire content of which is incorporated into this article by reference. Technical field [0003] The present invention generally relates to the field of integrated optics, and more specifically relates to an optical fluidic platform for optical particle detection without the need for advanced microscope equipment. The optical flow control platform may include a planar liquid core integrated optical waveguide for specific detection of nucleic acids. The optical waveguide can be an anti-resonance reflective optical waveguide, also known as ARROW or ARROW waveguide. Background technique [0004] Nucleic acid detection (NAT) is a major ...

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Application Information

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IPC IPC(8): C12Q1/68G01N33/52G01N33/50G01N21/17
CPCC12Q1/6818C12Q1/6825G01N21/03G01N21/6428G01N2021/0346B01L2200/0647B01L2200/12B01L2300/0816B01L2300/0819B01L2300/0887B01L2400/0415B01L3/502715C12Q2565/629G01N21/6486G01N21/645G01N2021/6439G01N2201/06113G01N2201/08
Inventor 霍尔格·施密特阿伦·罗·霍金斯
Owner RGT UNIV OF CALIFORNIA