Freeze-dried vaccine for porcine reproductive and respiratory syndrome and preparation method thereof

Abstract

The invention relates to a freeze-dried vaccine for porcine reproductive and respiratory syndrome and a preparation method thereof. The freeze-dried vaccine is prepared from a porcine reproductive and respiratory syndrome viral antigen and a protective agent, wherein the protective agent is prepared from the following substances by weight percent: 2-3% of NZ-Amine AS, 0.1-0.3% of glutamic acid mono potassium salt, 10-15% of cane sugar, 1-2% of lactoalbumin hydrolysate, 10-20% of hydrolyzed gelatin, 0.1-0.2% of vitamin C and the balance of water for injection. The heat-resisting protective agent is capable of minimizing injuries caused by various physical and chemical factors to virus activity during processes of sub-packaging and freeze-drying products, so that the titer loss of a virus before and after freeze-drying is reduced to be not more than 0.2 titer; and the live vaccine of the heat-resisting protective agent is high in anti-aging degree, the loss ratio of the virus does not exceed 0.5 titer after the vaccine is preserved for 10 days at the temperature of 37 DEG C, the titer reduction of the vaccine is little after the vaccine is preserved for 24 months at the temperature of 2-8 DEG C, and therefore, the problems of energy waste and preservation inconvenience caused by a strict cold chain condition required in transportation and preservation of conventional vaccines are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of livestock vaccine preparation, and in particular relates to a porcine reproductive and respiratory syndrome freeze-dried vaccine and a preparation method thereof. Background technique [0002] Lyoprotectants may affect the quality, potency and stability of biological products. Heat-resistant lyoprotectants have immunological activity but no pharmacological activity. It not only protects the biological activity of products, but also has excipients and antioxidants. The role of the vaccine is directly related to the titer and stability of the vaccine. It can maintain the stability of the vaccine during freeze-drying and storage, and ensure that the product can be stored at 2-8°C for up to 24 months. Even at 37°C, it can be stored for more than ten days, thus solving the problem of heat resistance, low storage temperature, short storage period and harsh freezing conditions caused by conventional vaccines dur...

AI Technical Summary

Problems solved by technology

In the process of vaccine production, transportation and use, if the cold chain system in any link is not properly controlled, the high temperature environment will lead to a decline in vaccine efficacy, and eventually cause epidemics due to immunization failure

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