Method for purifying lectin contained in peanut seeds by adopting hydroformylation hemocyte
A blood cell and lectin technology, applied in the field of aldehydized blood cell purification of lectin in peanut seeds, can solve the problems of low content, difficult purification, and obstacles to in-depth research, and achieve the effect of mild conditions and high recovery rate
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Embodiment 1
[0020] A method for purifying lectin in peanut seeds by formaldehyde blood cells, characterized in that: the prepared peanut powder is degreased with petroleum ether, mixed with formaldehyde blood cells, coupled, eluted and freeze-dried to obtain lectin ; The specific steps are as follows:
[0021] Preparation of lectin crude extract: Take peanut seeds without mildew and rot and insects, wash them with running water, dry them in a blast drying oven at 30°C, remove the peanut coats from the dried raw materials, crush them with a plant grinder, and pass the crushed materials through Use a 50-mesh sieve to collect the sieved material as peanut powder for extracting lectin; add 20 times (the mass ratio of peanut powder to petroleum ether) petroleum ether to peanut powder, shake it in a constant temperature water bath at 30°C for 5 hours, filter with suction, discard the filtrate, and keep the filter residue , after drying the filter residue in a fume hood, add 5 times the volume o...
Embodiment 2
[0025] Take fresh chicken blood and add anticoagulant for later use; resuspend the blood with anticoagulant added in twice the volume of sterilized phosphate buffered saline (PBS), centrifuge at 2000 rpm for 5 minutes, resuspend with PBS for 3 times, and finally remove the blood cells The specific volume was diluted to 0.05 for use; 10 times the volume of 0.05 blood cells was mixed with 1 times the volume of neuraminidase, washed 3 times with sterilized PBS, and resuspended to the specific volume of 0.2 enzyme-treated blood cells; 4 times the volume of 0.2 specific volume After the enzyme-treated blood cells reacted with 1 volume of aldylation agent for a certain period of time, they were washed with sterilized PBS for 3-4 times, and the blood cells were diluted with PBS to have a specific volume of 0.6. 4°C refrigerator for later use;
[0026] Take the peanut seeds without mildew and rot, wash them with running water, dry them in a blast drying oven at 30°C, remove the peanut ...
Embodiment 3
[0028] Take fresh duck blood and add anticoagulant for later use; then add twice the volume of sterilized phosphate buffer solution (PBS) to resuspend, centrifuge at 2000 rpm for 5 minutes, resuspend twice with PBS, and finally dilute the hematocrit to 0.05 Standby; 10 times the volume of 0.05 blood cells mixed with 1 times the volume of neuraminidase, washed 3 times with sterilized PBS, and resuspended to a specific volume of 0.2 enzyme-treated blood cells; 4 times the volume of 0.3 specific volume of enzyme-treated blood cells mixed with 1 times After reacting for a certain period of time with a certain volume of aldehylation agent, wash with sterilized PBS for 3 times, dilute the blood cells with PBS to the formaldehyde blood cells with a specific volume of 0.5, 4°C refrigerator for later use;
[0029] Take the peanut seeds without mildew and rot and moths, wash them with running water, dry them in a blast drying oven at 30°C, remove the peanut coats from the dried raw mater...
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