Tissue culture method for flame nandina

A technique for flaming N. chinensis and tissue culture, applied in the breeding field, can solve the problems affecting the industrialization development of flaming N. chinensis, slow propagation speed of flaming N. chinensis, short stem nodes and the like, and achieves good growth of new shoots and a proliferation cycle. The effect of short, stout stems

Inactive Publication Date: 2013-09-25
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the short stem nodes and few branches of the flame nandina, the propagation speed is too slow by the traditional cutting and branching methods, and it is difficult to form industrialized development. At present, the research on the tissue culture technology of flame nandina has just begun, and the propagation cycle is long , the problem of low reproduction coefficient has not been overcome, resulting in slow propagation speed of flame nandina and relatively lagging development speed, which seriously affects the industrialization development of flame nandina

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for rapid propagation of flame nandina tissue culture, comprising the following steps:

[0029] (1) Obtain sterile explants

[0030] In the spring, cut the newly germinated buds of Flaming Nantian bamboo, wash the materials with washing powder or liquid detergent, rinse them under tap water for 30 minutes, soak them in 75% ethanol for 20 seconds on an ultra-clean workbench, and then wash them with 0.l % mercuric chloride solution soaked for 8 minutes, rinsed with sterile water 6 times, and blotted the surface moisture.

[0031] (2) Primary culture

[0032] Take the base of the small buds, cut them into 1cm-long sections, inoculate them on the induction medium, control the culture temperature at 24-26°C, light for 16 hours a day, and the light is 30 mo1 / ms, and carry out bud induction culture; the small buds are inoculated on the small buds On the induction medium, the callus began to produce after 25 days, and the subculture was carried out after 1 month ...

Embodiment 2

[0041] A method for rapid propagation of flame nandina tissue culture, comprising the following steps:

[0042] (1) Obtain sterile explants

[0043] Cut the newly germinated buds of Flaming Nantian bamboo in spring, wash the material with washing powder or liquid detergent, rinse it under tap water for 30 minutes, soak it in 75% ethanol for 30 seconds on the ultra-clean workbench, and then wash it with 0.l % mercuric chloride solution soaked for 10 minutes, rinsed with sterile water 8 times, and blotted the surface moisture.

[0044] (2) Primary culture

[0045] Take the base of the small buds, cut them into 1cm-long sections, inoculate them on the induction medium, control the culture temperature at 24-26°C, light for 16 hours a day, and light at 40 mo1 / ms, and carry out bud induction culture; the small buds are inoculated on the small buds On the induction medium, the callus began to produce after 25 days, and the subculture was carried out after 1 month of continuous ...

Embodiment 3

[0054] A method for rapid propagation of flame nandina tissue culture, comprising the following steps:

[0055] (1) Obtain sterile explants

[0056] Cut the newly germinated buds of Flaming Nantian bamboo in spring, wash the materials with washing powder or liquid detergent, rinse them under tap water for 30 minutes, soak them in 75% ethanol for 30s on the ultra-clean workbench, and then wash them with 0.l % mercuric chloride solution soaked for 15 minutes, rinsed with sterile water 8 times, and blotted the surface moisture.

[0057] (2) Primary culture

[0058] Take the base of the small buds, cut them into 1cm long pieces, inoculate them on the induction medium, control the culture temperature at 24-26°C, light for 16 hours a day, and light at 40 mo1 / ms, and carry out bud induction culture; the small buds are inoculated on the small buds On the induction medium, the callus began to produce after 25 days, and the subculture was carried out after 1 month of continuous cu...

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Abstract

The invention relates to a tissue culture method for flame nandina, and belongs to the technical field of propagation. According to the method, new germinated buds of the flame nandina are used as explants. The method comprises the following steps of: obtaining germfree materials, primarily culturing the germfree materials, performing multiplication culture, performing rooting and seedling robusting culture, performing acclimatization and transplantation, and finally obtaining the flame nandina plants with high performance. Compared with the prior art, the method has the advantages that two culture mediums such as MS and WPM are mixed, so that the phenomena of low tissue culture seedling growth condition and low tissue culture seedling quality of the flame nandina are avoided; the rooting culture and the seedling robusting culture are simultaneously executed, so that a tissue culture procedure is saved; the production cost is saved; the transplantation survival rate is up to 90 percent; the flame nandina propagation speed and the quality of the seedlings are greatly improved; industrial development of the flame nandina is guaranteed.

Description

technical field [0001] The invention relates to a method for tissue culture of garden plants, in particular to a method for tissue culture of flame nandina, belonging to the technical field of reproduction. Background technique [0002] Flaming Nandina belongs to the genus Nandina of the family Nandinaceae. The plant is short and compact, with oval or ovate leaves and extremely short internodes, only 1 / 10 of ordinary nandina. Flame nandina begins to germinate in mid-April in Shanghai area, and grows tender red new leaves. Big, when the temperature rises, it will gradually turn green above 30°C. In December, when the temperature drops below 5°C and the temperature difference between day and night is above 10°C, the leaves begin to turn red. Because it is cold-resistant and evergreen, it will not wither through winter and is very beautiful. , suitable for ground cover and small garden environment layout. The research on the tissue culture technology of flame nandina has only...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 王姗王永平鲍华鹏
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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