Determination method of potency of traditional Chinese medicine zedoary turmeric oil by using metabolomics

Abstract

The invention discloses a determination method of the potency of a traditional Chinese medicine zedoary turmeric oil by using metabolomics. The method comprises the steps that: an unilateral ureteral obstruction induced renal interstitial fibrosis model is adopted, an animal modeling is carried out; processing and sampling are carried out after animal modeling is carried out for 3 days, 7 days, 14 days, 21 days, and 28 days; animal kidney tissues are fetched; kidney tissue pathological changes are observed by using an optical microscope; serum samples are prepared, and 1H NMR spectral data is obtained; NMR data processing and pattern recognition analysis are carried out; and analysis is carried out by using SPSS13.0 software. According to the invention, information of large amount of endogenous metabolites in bodies can be obtained with the metabolomic technology, and metabolite changes of potencies of various effective components in zedoary turmeric oil upon various targets can be screened. Therefore, the potency of zedoary turmeric oil can be comprehensively evaluated. The invention provides an excellent method for evaluating zedoary turmeric oil potency.

Description

technical field [0001] The invention belongs to the technical field of research on curcuma oil, a traditional Chinese medicine, and in particular relates to a method for measuring the efficacy of curcuma oil, a traditional Chinese medicine, by applying metabolomics. Background technique [0002] Zedoary oil has a wide range of pharmacological activities and can be used in the fields of anti-tumor, anti-virus, anti-bacteria and anti-inflammation. However, due to historical limitations, the therapeutic effect of traditional Chinese medicine on the warming of Zezhu often stays in the summary of experience and macroscopic description, and lacks the basis of scientific experiments. To explain and verify, the mechanism of action of zedoary oil should be "multi-component, multi-target" in theory, but it often lacks effective research methods and technical means to prove it. The characteristics of the function of the body bring great difficulties to the study of the exact molecular ...

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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a method for measuring the efficacy of traditional Chinese medicine zedoary oil by applying metabolomics, aiming to solve the problem that there are many active ingredients in warming zedoary oil and having multiple drug effects ;Each active compound component may have multiple targets and directly affect multiple biological processes; there is synergy and mutual influence between components, and the ideological basis of traditional Chinese medicine compatibility is synergy. A single chemical component and pharmacological index The problem of not being able to clearly understand the drug effect of warm curcuma

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