Early tobacco virus disease detection method based on infrared thermal imaging technology
A technology of infrared thermal imaging and tobacco virus, which is applied in the early detection of tobacco virus disease. The use of infrared thermal imaging technology in the field of early detection of tobacco virus disease can solve the problems of poor taste, damage, and quality decline, and achieve objective Strong performance, high accuracy, and simple steps
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Embodiment 1
[0022] 1. 60 tobacco plants with the same plant height, leaf age and growth were selected, and the lower old leaves were cut off from each plant, leaving 4 upper leaves. 30 plants were the control group and 30 plants were the inoculation group. Use 10 times the volume (V / W) of 0.02mol / L phosphate buffer solution, pH 7.0, to grind fresh diseased leaves infected with TMV, PVY and CMV3 viruses, filter with gauze and take the supernatant, and use the conventional friction inoculation method The fourth leaf of tobacco from top to bottom was inoculated, and the control group was smeared with 0.02mol / L phosphate buffer solution, pH 7.0, labeled and recorded. The two groups of plants were placed separately in the greenhouse of plant light cultivation racks for cultivation, with a light intensity of 10,000 lux, a temperature of 18±2°C, and a humidity of 70±10%.
[0023] 2. At 9:00 in the morning, the tobacco plants were placed in the measurement environment to balance for 60 minutes,...
Embodiment 2
[0026] 1. 50 tobacco plants with the same plant height, leaf age and growth were selected, and the lower old leaves were cut off from each plant, leaving 4 upper leaves. 25 plants were the control group and 25 plants were the inoculation group. Use 10 times the volume (V / W) of 0.02mol / L phosphate buffer solution, pH 7.0, to grind fresh diseased leaves infected with TMV, PVY and CMV3 viruses, filter with gauze and take the supernatant, and use the conventional friction inoculation method The fourth leaf of tobacco from top to bottom was inoculated, and the control group was smeared with 0.02mol / L phosphate buffer solution, pH 7.0, labeled and recorded. The two groups of plants were placed separately in the greenhouse of plant light cultivation racks for cultivation, with a light intensity of 10,000 lux, a temperature of 32±2°C, and a humidity of 70±10%.
[0027] 2. At 9:00 in the morning, the tobacco plants were placed in the measurement environment to balance for 60 minutes,...
Embodiment 3
[0030] 1. 70 tobacco plants with the same plant height, leaf age and growth were selected, and the lower old leaves were cut off from each plant, leaving 4 upper leaves. 35 plants were the control group and 35 plants were the inoculation group. Use 10 times the volume (V / W) of 0.02mol / L phosphate buffer solution, pH = 7.0, grind fresh diseased leaves infected with TMV, PVY and CMV3 viruses, filter with gauze, take the supernatant, and use the conventional friction inoculation method Inoculate the fourth leaf of tobacco from top to bottom, apply 0.02mol / L phosphate buffer solution, pH=7.0, to the control group, label it, and make a record. The two groups of plants were placed separately in the greenhouse of plant light cultivation racks for cultivation, with a light intensity of 10,000 lux, a temperature of 26±2°C, and a humidity of 70±10%.
[0031] 2. At 9:00 in the morning, the tobacco plants were placed in the measurement environment to balance for 120 minutes, and thermal...
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