Method of using biotransformation of rice straws and peanut cakes to prepare composite enzyme and probiotics preparation for forage

A technology of probiotic preparation and rice straw, which is applied in animal feed, animal feed, application, etc., can solve the problems of difficult digestion of monogastric animals, and achieve the effect of low-cost, high-efficiency biotransformation, and strong enzyme production capacity

Active Publication Date: 2013-10-09
JINHUA ACAD OF AGRI SCI
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of rice straw, 5kg of peanut cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and cultivate it at 35°C for 48 hours to form a starter;

[0027] 2) Fully mix 20kg of the starter in step 1) with 600kg of rice straw, 300kg of peanut cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, ferment at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0028] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 45.22IU / g; total cellulase activity reaches 3.97IU / g; beta-glucosidase activity reaches 3.97IU / g; reached 9.45IU / g; endoglucanase activity reached 38.72IU / g; xylanase activity reached 46.94IU / g; beta-xylosidase activity reached 3.12IU / g; polyga...

Embodiment 2

[0030] 1) Fully mix 10kg of fermented Pu-erh ripe tea with 10kg of rice straw, 5kg of peanut cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate it at 35°C for 48 hours to form a starter;

[0031] 2) Fully mix 20kg of the starter in step 1) with 600kg of rice straw, 300kg of peanut cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, ferment at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0032] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 45.34IU / g; total cellulase activity reaches 4.03IU / g; beta-glucosidase activity reaches 45.34IU / g; reached 9.76IU / g; endoglucanase activity reached 38.97IU / g; xylanase activity reached 47.14IU / g; beta-xylosidase activity reached 3.34IU / g; polygal...

Embodiment 3

[0034] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of rice straw, 5kg of peanut cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate it at 40°C for 36 hours to form a starter;

[0035] 2) Fully mix 10kg of the starter in step 1) with 600kg of rice straw, 300kg of peanut cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, natural ventilation, and ferment at 35°C for 5 Day, get the compound enzyme and probiotic preparation for feed.

[0036] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 46.66IU / g; total cellulase activity reaches 3.88IU / g; beta-glucosidase activity reaches 3.88IU / g; up to 10.15IU / g; endoglucanase activity up to 40.26IU / g; xylanase activity up to 45.85IU / g; beta-xylosidase activity up to 3.20IU / g; polygalacturonase a...

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Abstract

A disclosed method of using biotransformation of rice straws and peanut cakes to prepare a composite enzyme and a probiotics preparation for a forage comprises the following steps: fully mixing Pu'er cooked tea after pile fermentation, first rice straws, first peanut cakes, first wheat bran, first apple pomaces and first water, domestication culturing and forming a starter; and fully mixing the starter, second rice straws, second peanut cakes, second wheat bran, second apple pomaces and second water, and fermenting to obtain the composite enzyme and the probiotics preparation for the forage. The composite enzyme and the probiotics preparation for the forage with various better performance indexes, is prepared by using a microecology-stable foodstuff-grade-safe flora to ferment the rice straws and the peanut cakes, and the low-cost high-efficiency biotransformation of the rice straws and the peanut cakes is realized. At the same time, the method of the invention has the advantages that the microbes are easy to grow, enzyme vitality is high, the fermentation process is extensive, no strict aseptic condition is needed, and the industrialized large-scale production is easy to realize.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting rice straw and peanut cake by adopting micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellu...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14
CPCY02P60/87
Inventor 阮晖项云徐志钱春芳陈功诸文颖纪晓燚吴渊杜姗姗杨璐
Owner JINHUA ACAD OF AGRI SCI
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