Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of using biotransformation of corncobs and rapeseed cakes to prepare composite enzyme and probiotics preparation for forage

A technology of rapeseed cake and probiotic preparations, which is applied in animal feed, animal feed, application, etc., can solve the problems of difficult digestion of monogastric animals, and achieve industrialized large-scale production, high enzyme activity, and easy growth of microorganisms Effect

Active Publication Date: 2014-12-03
JINHUA ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of corncobs, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and cultivate them at 35°C for 48 hours to form a starter;

[0027] 2) Fully mix 20kg of the starter in step 1) with 600kg of corncobs, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0028] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 78.83IU / g; total cellulase activity reaches 6.27IU / g; beta-glucosidase activity reaches 6.27IU / g; reached 14.82IU / g; endoglucanase activity reached 58.34IU / g; xylanase activity reached 78.45IU / g; beta-xylosidase activity reached 5.06IU / g; polyga...

Embodiment 2

[0030] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of corncobs, 5kg of rapeseed meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate them at 35°C for 48 hours to form a starter;

[0031] 2) Fully mix 20kg of the starter in step 1) with 600kg of corncobs, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0032] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 78.75IU / g; total cellulase activity reaches 6.34IU / g; beta-glucosidase activity reaches 78.75IU / g; reached 14.69IU / g; endoglucanase activity reached 58.37IU / g; xylanase activity reached 78.53IU / g; beta-xylosidase activity reached 5.14IU / g; polyg...

Embodiment 3

[0034] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of corncobs, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate them at 40°C for 36 hours to form a starter;

[0035] 2) Fully mix 10kg of starter in step 1) with 600kg of corncobs, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0036] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 70.45IU / g; total cellulase activity reaches 6.23IU / g; beta-glucosidase activity reaches 70.45IU / g; reached 13.96IU / g; endoglucanase activity reached 60.42IU / g; xylanase activity reached 71.24IU / g; beta-xylosidase activity reached 4.88IU / g;...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A disclosed method of using biotransformation of corncobs and rapeseed cakes to prepare a composite enzyme and a probiotics preparation for a forage comprises the following steps: fully mixing Pu'er cooked tea after pile fermentation, first corncobs, first rapeseed cakes, first wheat bran, first apple pomaces and first water, domestication culturing and forming a starter; and fully mixing the starter, second corncobs, second rapeseed cakes, second wheat bran, second apple pomaces and second water, and fermenting to obtain the composite enzyme and the probiotics preparation for the forage. The composite enzyme and the probiotics preparation for the forage with various better performance indexes, is prepared by using a microecology-stable foodstuff-grade-safe flora to ferment the corncobs and the rapeseed cakes, and the low-cost high-efficiency biotransformation of the corncobs and the rapeseed cakes is realized. At the same time, the method of the invention has the advantages that the microbes are easy to grow, enzyme vitality is high, the fermentation process is extensive, no strict aseptic condition is needed, and the industrialized large-scale production is easy to realize.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting corn cobs and rapeseed cakes with micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellulose,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖项云徐志钱春芳陈功诸文颖纪晓燚吴渊杜姗姗杨璐
Owner JINHUA ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com