Bacillus and application of bacillus in exopolysaccharides preparation

A technology for bacillus and polysaccharide, which is applied to the production of natural exopolysaccharide strains and natural exopolysaccharide, in the field of bioengineering, can solve problems such as no existing, and achieves the effects of low production cost, simple production process and easy extraction.

Active Publication Date: 2013-10-16
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report in the field of polysaccharide production and metabolism of Bacillus tequilensis (Bacillus tequilensis)

Method used

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  • Bacillus and application of bacillus in exopolysaccharides preparation
  • Bacillus and application of bacillus in exopolysaccharides preparation
  • Bacillus and application of bacillus in exopolysaccharides preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment one: bacillus tequila ( Bacillus tequilensis ) Screening, classification and identification of Be14-2 CGMCC No.7647

[0029] The Bacillus Be14-2, which was isolated and screened from desert soil samples in Xinjiang, was identified as Bacillus Tequila ( Bacillus tequilensis ). At present, the strain has been deposited in the International Depository of Microorganisms under the Budapest Treaty: the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms before the application date. Address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip Code: 100101, the deposit date is May 27, 2013, and the deposit number is CGMCC No.7647.

[0030] Bacterial strain Tequila bacillus provided by the invention ( Bacillus tequilensis ) Be14-2 CGMCC No.7647, grown on Chapei's medium, the colonies are small, flat or slightly raised, viscous; the diameter of the ...

Embodiment 2

[0035] Embodiment two: bacillus tequila ( Bacillus tequilensis ) Tolerance test of Be14-2 CGMCC No.7647 to heavy metal ions

[0036] The strain Bacillus tequila ( Bacillus tequilensis ) Be14-2 CGMCC No.7647 were inoculated in different concentrations of Pb 2+ 、Cu 2+ 、Co 2+ , Hg 2+ , Zn 2+ On the PDA solid medium plate, culture at 30 ℃ constant temperature, detect its growth status. The measurement results are shown in Table 2.

[0037] Table 2 Tolerance test of Be14-2 strain to different heavy metals (concentration unit mg / L)

[0038] 　 50 100 200 300 400 500 600 700 800 900 1000 1100 2000 Pb 2+ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ Cu 2+ ﹢ ﹢ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ co 2+ ﹢ ﹢ ﹢ + + - - - - - - - - Hg 2+ ﹢ ﹢ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ ﹣ Zn 2+ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ ﹢ - ﹣ ﹣ ﹣

[0039] Note: ﹢ means growth, - means no growth

[0040] From the results in Table 2, it can be kno...

Embodiment 3

[0041] Example Three: Polysaccharide Extraction and Its Ultraviolet Absorption Properties

[0042] Bacillus tequila ( Bacillus tequilensis ) Be14-2 CGMCC No.7647 was placed into a 500ml Erlenmeyer flask containing 200ml of Chase liquid medium with 10% inoculation amount, fermented at 37°C and 180rpm on a shaking table for 48h, and the pH value of the fermentation broth was adjusted to 11.0, and centrifuged at 10000rpm for 5min to remove The bacterial cell was deproteinized by the Sevag method, and 95% ethanol was added to precipitate with 3 times the volume, and then dried to obtain the crude exopolysaccharide extract of the bacterial cell.

[0043] The crude polysaccharide extract is a white solid, 0.1 g is weighed and dissolved in 100 ml of water, mixed well, the pH is determined to be 6.5, and then analyzed by light scanning at 200-800 nm.

[0044] The experimental results show that the polysaccharide has a specific absorption peak at 200-300nm, see the attached Figur...

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Abstract

The invention discloses bacillus tequilensis Be14-2 CGMCC No. 7647 (China General Microbiological Culture Collection Center Number 7647), and an application of the bacillus tequilensis in polysaccharide metabolism. The bacillus tequilensis is inoculated in a czapek medium for inoculated cultivation, so that Pb<2+> tolerance is 2000mg / L, maximum Zn<2+> tolerance is 800mg / L, maximum Co<2+> tolerance is 400mg / L, and maximum Cu<2+> tolerance and maximum Hg<2+> tolerance are 100mg / L; a fermented culture can directly serve as an antioxidant; a concentration of polysaccharide with 50% hydroxyl radical (*OH) removed is 13.47mg / ml; the fermented culture can also be used for flocculating; and an activity range of the flocculating is 75.32-84.73%. The bacillus tequilensis is widely applied to the field of the polysaccharide metabolism, provides a new approach for industrially producing the natural polysaccharide, and has an important effect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. The invention specifically relates to the technical field of a bacillus and its application in the preparation of exopolysaccharides. Further, the invention relates to production strains, polysaccharides and preparation methods of natural exopolysaccharides, in particular to The technical field of strains producing natural exopolysaccharides and natural exopolysaccharides. Background technique [0002] Polysaccharides are a large class of biomacromolecules ubiquitous in living organisms. In addition to nutritional effects, they also have special biological activities and pharmacological properties, such as immune regulation, anti-tumor, anti-mutation, lowering blood lipids, anti-virus, and anti-aging , anti-oxidation and anti-rheumatic arthritis and other functions, can be widely used in food and pharmaceutical industries, as well as industrial wastewater treatment, cleaning supplies, text...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/07
Inventor 宋素琴张志东房世杰王玮顾美英朱静高雁乔坤云
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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