Pre-treatment method for measuring nitrogen and phosphorus in cells of algae in water

Abstract

The invention relates to a pre-treatment method for measuring nitrogen and phosphorus in cells of algae in water. The pre-treatment method comprises: sampling a water sample, adopting a pre-treated micropore glass fiber filtration membrane to filter the sampled water sample, enriching algae cells in the water sample on the filtration membrane, placing the micropore glass fiber filtration membrane enriching and trapping the algae cells in a color comparison tube having a stopper, adding distilled water and an oxidant to carry out digestion, filtering after digestion, collecting the filtrate, carrying out volume metering in the color comparison tube having a stopper, and concurrently carrying out nitrogen content and phosphorus content measurement on the volume-metered liquid. According to the present invention, the new oxidant is introduced, such that nitrogen and phosphorus in algae cells can be digested in the same process so as to reduce the required amount of the sample, and reduce sampling time requirements and sampling cost, wherein the less required amount of the sample can provide the following advantages that: the experiment time can be effectively reduced, sample analysis completion within a short time can be ensured, and change of sample quality due to long time sample placing can be avoided so as to substantially improve experiment efficiency and reduce experiment material consumption cost and labor cost.

Description

technical field [0001] The invention relates to the field of chemical analysis, in particular to a pretreatment method for measuring the content of nitrogen and phosphorus elements in algae cells in water. Background technique [0002] Algae is a tiny plant that lives in suspension in water. It is widely distributed in oceans and freshwater bodies around the world, and plays an important role in the biogeochemical cycle of biogenic elements. In recent years, the research work of algae metrology has been extensively carried out in the research of freshwater ecology and biogeochemistry. It is necessary to analyze the element content or material composition of algae cells in the water body, so as to clarify the influence of element composition in algae cells on environmental changes. response process. Nutrient elements such as nitrogen and phosphorus are the main substances that affect the growth of algae, and are the main focus of quantitative chemistry research. [0003] Th...

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Problems solved by technology

Repeated sampling not only increases the demand for samples, but also increases the cost of sample collection and pretreatment

[0006] 2) At present, the oxidants used in the general nitrogen and phosphorus digestion process are different. The digestion of nitrogen and phosphorus needs to be carried out separately, which increases the consumables and time consumption of indoor pre-treatment, and also increases the manpower and physical input.

[0007] 3) For samples collected in the same area, due to the temporal and spatial variability of the environment, it is difficult to ensure complete consistency in sampling when measuring nitrogen and phosphorus content respectively

Due to the heterogeneity of the sample itself and the differences caused by the operation during the sampling process, it is difficult to overcome, resulting in large deviations in the measurement results of nitrogen and phosphorus

Especially when elemental analyzer is used for trace analysis of nitrogen and digestion test is used for phosphorus, the detection limit and sensitivity of the methods used by the two sets of indicators are not in the same order of magnitude, and the error of the obtained results will inevitably have an order of magnitude difference. It has caused great obstacles to the accurate analysis of nitrogen and phosphorus content in algae cells

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