Ice-temperature organ protection solution

Abstract

The invention discloses an ice-temperature organ protection solution. A sterilizing aqueous solution is prepared from potassium citrate, sodium citrate, magnesium sulfate, mannitol, 6-aminoadenine phosphate, astragalus membranaceus and water for injection by mixing. Every 100ml of the ice-temperature organ protection solution comprises 0.87-1.16g of potassium citrate, 0.98-1.19g of sodium citrate, 1.02-1.15g of magnesium sulfate, 5.56-6.85g of mannitol and 0.45g of 6-aminoadenine phosphate and 5ml of astragalus membranaceus extract. The ice-temperature organ protection solution is prepared by mixing the potassium citrate, the sodium citrate, the magnesium sulfate, the mannitol, the 6-aminoadenine phosphate and the astragalus membranaceus, and adding the water for injection into a 100ml bottle to dilute the volume to 100ml. Because the proportion of the mannitol is increased, the phase-transition temperature point of the ice-temperature organ protection solution can be decreased, and the ice-temperature organ protection solution is more suitable for preserving an organ at ice temperature. The added astragalus membranaceus has the functions of resisting the oxidative damage and promoting the activity of a Na<+>-K<+>-ATP enzyme. The ice-temperature organ protection solution has low viscosity and a good perfusion effect and can decrease the temperature of an organ rapidly in a short time.

Description

technical field [0001] The invention belongs to a protective liquid for organ preservation, in particular to an organ protective liquid suitable for freezing temperatures. Background technique [0002] Organ preservation is one of the three technical pillars of organ transplantation. Due to the emergence of organ protection solutions, organ preservation technology has made great progress in the past 10 years. An ideal preservation solution can provide a suitable microenvironment for isolated organs and maximize the preservation time of organs. It has always been a research hotspot in the field of organ transplantation. The most commonly used clinically is the UW solution (University of Wisconsin Solution) developed by the University of Wisconsin. Due to its good clinical and experimental preservation effects, this preservation solution is considered as a standard organ preservation solution, but its price is relatively expensive, which limits its wide application in the fie...

AI Technical Summary

Problems solved by technology

Although UW fluid basically meets the above conditions, in addition to complex components and high price, it also has high fluid viscosity, slow cooling of organs, poor buffering capacity, K + Defects such as high content

For this reason, a series of organ protection solutions have also been developed in China, but the storage adaptation temperature of the above-mentioned protection solutions is in the range of 0-4°C, and the upper limit is taken when using them.

[0003] As we all know, the lower the temperature is, the more favorable it is for biological preservation, but the lower the temperature, the microtubule-associated protein (MAP) loses the ability to bind microtubules, and the lipid of the cell membrane changes from a liquid crystal state to a gel state, resulting in the fluidity of the cell membrane. decrease, disrupting the balance of osmotic pressure inside and outside the cell