Dyetree fruit polyphenol extract with antineoplastic effect and preparation method thereof
A technology of anti-tumor effect and fragrant fruit, which is applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., to achieve the effect of light color, simple preparation method and high purity
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Embodiment 1
[0020] (1) 150 g of the infructescences of the fragrant tree were used as raw material, after drying and crushing, 400 mL of petroleum ether solution was added to extract at room temperature for 10 h to degrease. Filter and dry the filter residue at 45°C;
[0021] (2) Add 400 mL of 70% methanol solution to the degreased infructescence raw material, extract in 40°C water bath for 3 times, each time for 1 h, filter, and combine the filtrates. The filtrate was removed by rotary evaporation under reduced pressure at 40°C to remove the methanol solvent, and the aqueous phase was freeze-dried to obtain 14.6 g of crude extract;
[0022] (3) The crude extract was dispersed in 100 mL of pure water, extracted three times with the same volume of ethyl acetate, and separated to obtain the ethyl acetate extract phase and the water phase;
[0023] (4) Evaporate the ethyl acetate extract phase under reduced pressure to remove the solvent, and freeze-dry to obtain 3.42 g of the light yellow ...
Embodiment 2
[0025] (1) 120 g of the infructescences of the fragrant tree were used as the raw material, after drying and crushing, 300 mL of petroleum ether solution was added to extract at room temperature for 4 h to degrease. Filter and dry the filter residue at 45°C;
[0026] (2) Add 400 mL of 80% methanol solution to the degreased infructescence raw material, extract in a water bath at 45°C for 3 times, each time for 1 h, filter, and combine the filtrates. The filtrate was removed by rotary evaporation under reduced pressure at 50°C to remove the methanol solvent, and the aqueous phase was freeze-dried to obtain a crude extract;
[0027] (3) The crude extract was dispersed in 100 mL of pure water, extracted three times with the same volume of ethyl acetate, and separated to obtain the ethyl acetate extract phase and the water phase;
[0028] (4) Evaporate the ethyl acetate extract phase under reduced pressure to remove the solvent, and freeze-dry to obtain 1.91 g of the light yellow ...
Embodiment 3
[0030] (1) 200 g of the infructescences of the fragrant tree were used as the raw material, after drying and crushing, 400 mL of petroleum ether solution was added to extract at room temperature for 2 h to degrease. Filter and dry the filter residue at room temperature;
[0031] (2) Add 700 mL of 60% methanol solution to the degreased infructescence raw material, extract in 40°C water bath for 3 times, each time for 1 h, filter, and combine the filtrates. The filtrate was removed by rotary evaporation under reduced pressure at 50°C to remove the methanol solvent, and the aqueous phase was freeze-dried to obtain a crude extract;
[0032] (3) The crude extract was dispersed in 100 mL of pure water, extracted three times with the same volume of ethyl acetate, and separated to obtain the ethyl acetate extract phase and the water phase;
[0033] (4) Evaporate the ethyl acetate extract phase under reduced pressure to remove the solvent, and freeze-dry to obtain 4.10 g of the light ...
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