Chinese medicinal mixed preparation for tumors
A technology of traditional Chinese medicine and preparation, applied in the field of mixed preparations of traditional Chinese medicine, can solve the problems of Chinese toon and musk, etc., and achieve the effect of inhibiting tumor cell growth, increasing lethality, and clear mechanism
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Embodiment 1
[0025] Embodiment 1. The preparation of Chinese medicine mixed reagent
[0026] Take musk powder and Chinese toon tree root bark powder, mix and boil according to the mass ratio of 1:20-1:50, and obtain a jelly-like effective extract, which is a mixed preparation of traditional Chinese medicine. The above-mentioned preparations are not added or used in production and treatment. any chemical composition. Get musk powder and Chinese toon root bark powder mass ratio 1:50 in the embodiment of the present invention.
Embodiment 2
[0027] Embodiment 2. The experiment that the mixed preparation of traditional Chinese medicine inhibits cancer cells
[0028] 1. Materials
[0029] Cervical cancer cell HeLa, mouse embryonic fibroblast NIH3T3, human liver cancer cell SMMU-7721, and human normal liver cell QSG-7701 were preserved and cultured in the laboratory; RPMI1640 dry powder, fetal bovine serum, and trypsin (containing EDTA) were purchased from Gibco The company; the cell counting kit (CCK-8) was purchased from Tongren Chemical Technology (Shanghai) Co., Ltd.; the cycle-related gene chip was ordered from Changzhou Chutian Biotechnology Co., Ltd.
[0030] 2. Cell culture
[0031] Cells were cultured in RPMI1640 medium with 10% serum and 1% double antibodies (penicillin, streptomycin). Cells were placed in 5% CO 2 , Cultivated in a 37°C incubator, subcultured every 2 to 3 days, and the cells in the logarithmic growth phase were used for experiments.
[0032] 3. Cytotoxicity assay
[0033] Cells in the ...
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