Novel biological endocranium patch

A kind of dural and biological technology, applied in the field of new biological dural patches, can solve the problems of increasing operation and treatment time, scar formation or wrapping reaction, and low immune response, so as to increase postoperative infection rate and reduce hematoma formation And mechanical formation of scars, the effect is satisfactory

Inactive Publication Date: 2013-10-23
成都达普医疗器械有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, artificial synthetic materials can cause serious rejection reactions, and due to local tissue reactions, they can cause brain tissue irritation, scarring or encapsulation reactions, meningitis symptoms or bleeding and other serious complications. Most repair materials rely on imports, which are expensive and difficult to promote in clinical practice; although autologous tissue repair materials have good biocompatibility and low immune respon

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Preparation of Type I Collagen Fiber Dura

[0021] 1.1 Raw material thawing:

[0022] The collagen is taken out from the freezer, and the usage amount of the batch is calculated according to the water content, placed in a sterilized plate, and used after thawing at room temperature.

[0023] 1.2 Preparation calculation method:

[0024] 0.05M, 600ml glacial acetic acid solution containing 3-5g dry collagen

[0025] 1.3 Preparation of type I collagen solution. Weigh 9.1-15.2g of the thawed collagen according to the water content and put it into a stirring tank, add 600-800ml of 0.05M glacial acetic acid, and stir for 1-2 hours.

[0026] 1.4 Freeze-drying: Put the solution in 1.3 into a plate, pour in different thicknesses of type I collagen solution according to clinical needs, and perform freeze-drying according to the following procedures:

[0027] step number

[0028] 1.5 Irradiation sterilization: Put the freeze-dried type I collagen fiber membrane mat...

Embodiment 2

[0029] Example 2 porosity measurement

[0030] Adopt the method of embodiment 1 to prepare 4 kinds of products with different irradiation intensities, adopt electron microscope to measure the pore size in its structure to be respectively 345um, 220um; Most suitable for the growth of fibroblasts.

Embodiment 3

[0031] The mensuration of embodiment 3 collagenase enzymolysis time

[0032] Using a self-made puncher, 4 kinds of dura mater substitute materials with different irradiation times were made into 10 discs each with a diameter of 5 mm, and then the small discs, enzymatic hydrolysis buffer and collagenase solution with an enzyme activity unit of 357 U / mL were added. In a small test tube, the time required for enzymatic hydrolysis at a constant temperature of 37°C to the decomposition of the small disc is the enzymatic hydrolysis time (min). The measured enzymatic hydrolysis times are: 25.3min, 23.6min, 27.6min, 51.1min. When the irradiation dose is 25-40KGy, the enzymolysis time of type I collagen fibrous membrane has little difference, which proves that its effect on collagen The degree of cross-linking has little effect.

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PUM

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Abstract

The invention discloses a novel biological endocranium patch which is prepared from I-type collagen, has a unique fiber structure, can absorb cerebrospinal fluid without increasing the volume, has a nutrition protection effect on nervous tissues, is good in biocompatibility, and can induce the growth of fibroblast cells and has an effect of guiding the regeneration of tissues, and meanwhile, an endocranium repairing method is simple and practicable, well in effect, and cannot increase the postoperation infection rate, so that the novel biological endocranium patch is an effective endocranium repairing material.

Description

technical field [0001] The invention relates to a novel biological dura mater patch, in particular to a freeze-dried collagen dura mater patch and application thereof. Background technique [0002] The dura mater is an important barrier to protect brain tissue, and is of great significance for maintaining the structure and functional activities of the nervous system. Trauma or craniocerebral surgery can cause dura mater defect. If it is not repaired, the wound will not heal easily, and complications such as subcutaneous fluid, cerebrospinal fluid leakage, and intracranial infection will occur; bleeding from the scalp or muscle layer can spread to the arachnoid The lower space stimulates the brain tissue, causing fever, headache, aseptic inflammation or meningeal irritation; the direct adhesion and healing of the brain tissue and the scalp increases the chance of epilepsy. Surgery to repair the dura mater and seal the subdural space can significantly reduce or prevent compli...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/58
Inventor 彭红卫李好雨王伟李鹏徐小萍毛庆刘文科
Owner 成都达普医疗器械有限公司
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