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Lactococcus lactis strains for use in improving digestive condition

A technology of Lactococcus lactis and strain, applied in the field of compositions of lactic acid bacteria, can solve the problems of increasing epithelial permeability, deterioration of epithelial barrier function and the like

Active Publication Date: 2013-10-23
DANONE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0028] It has also been shown that during the active phase of IBD, inflammation of the gastrointestinal tract causes increased epithelial permeability leading to deterioration of epithelial barrier function (Madsen et al., 2001)

Method used

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  • Lactococcus lactis strains for use in improving digestive condition
  • Lactococcus lactis strains for use in improving digestive condition

Examples

Experimental program
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Effect test

Embodiment 1

[0081] Example 1: Evaluation of the antipathogenic activity of Lactococcus lactis strain DN030066

[0082] Antimicrobial activity against Salmonella Enteritidis B1241, Listeria and pathogenic E. coli was determined in an overlay assay. Three Lactococcus lactis strains were evaluated in this test: strain DN030066 according to the invention, Lactococcus lactis subsp. lactis DSMZ20729 and Lactococcus lactis subsp. Cremoris DSMZ4645 previously described as bacteriocin producing strains (nisin and nisin, respectively) (Bartoloni et al., 2004 and Park et al., 2003).

[0083] Lactococcus lactis strains were transferred from frozen stocks to fresh agar plates using stamps. Plates were incubated overnight until visible colonies were observed. Subsequently, top agar containing dilutions of brain heart infusion broth and selected pathogens were poured onto agar plates. Plates were incubated at 37°C. The next day, measure the diameter of the pathogen inhibition zone.

[0084] A sco...

Embodiment 2

[0088] Example 2 : TEER Evaluation of Lactococcus lactis Strains DN030066 and DN030087

[0089] Intestinal barrier integrity can be assessed by measuring the potential difference observed between the apical and basal surfaces of the T84 cell monolayer. This experimental model is called transepithelial electrical resistance (TEER) (Hirotani et al., 2008).

[0090] Culture suspensions of different bacterial strains were washed with PBS. Subsequently, bacteria (100 cfu / cell) were added on top of the T84 cell monolayer. 96 strains were tested in this assay, including 64 Bifidobacteria, 32 Lactobacilli and 2 Lactococcus lactis (DN0300664 and DN030087). After 4 hours and 6 hours of incubation with different bacteria, TEER values ​​were measured to evaluate the epithelial barrier function. All experiments were performed 3 times independently and in triplicate. Set the value at 100% for T84 cells at t = 0. The results for the 7 strains with higher TEER values ​​are shown in T...

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Abstract

The present invention concerns strains of Lactococcus lactis capable of inhibiting the growth of a pathogenic microorganism and / or improving the intestinal epithelial barrier integrity. These strains are suitable for use in the treatment or prevention of a digestive disorder.

Description

technical field [0001] The present invention relates to a composition comprising lactic acid bacteria for improving digestive conditions. In particular, such compositions comprise Lactococcus lactis strains suitable for the treatment and / or prevention of digestive diseases. Background technique [0002] Gastrointestinal infections are extremely common around the world. In the vast majority of affected people, full recovery occurs rapidly. However, a large proportion of patients with gastroenteritis develop long-term gastrointestinal symptoms such as irritable bowel syndrome (IBS). Indeed, it is estimated that 3.7 to 36% of individuals develop IBS following bacterial or viral gastroenteritis (Spiller and Garsed, 2009). [0003] Gastrointestinal infections are also among the most common causes of diarrhea. In severe cases, the infection that caused the diarrhea can further cause inflammation of the stomach. [0004] Gastrointestinal infections also include Crohn's disease...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P1/00
CPCA61K35/742A61K35/74A61P1/00A61P1/04A61P1/10A61P1/12A61P1/14Y02A50/30A23C9/12C12N1/20
Inventor 比里亚纳·莱西奇伊莎贝尔·尚博
Owner DANONE
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