Acanthus ilicifolius plant tissue culture medium and preparation method thereof

A plant tissue and culture medium technology, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of not being able to meet large-scale production, market demand, market demand, etc., and achieve shortened cultivation The effect of cycle, fast reproduction speed and low cost

Inactive Publication Date: 2013-10-30
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual production, the mouse bougainvillea generally adopts the seed propagation method. Although the seed propagation technology is widely used, there are still many shortcomings, such as time-consuming and labor...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Example 1, taking the preparation of 1L differentiation medium as an example, weigh 1L of MS, 1mg of BA, 0.2mg of NAA, 500mg of L-proline, 500mg of L-glutamine, 200mg of Hydrolyzed casein, 3000mg of sucrose, 5500mg of agar powder, first add agar powder to a pot with 0.8L of distilled water and boil, then add MS, L-proline, L-glutamine, hydrolyzed casein, BA to the pot , NAA, after boiling, add sucrose, and after the sucrose melts, adjust the volume to 1L, adjust the pH value to 5.8, then put the culture medium after the volume into the container for autoclaving, and the sterilization temperature is 121°C , the pressure is 0.1kPa, cooled to 1 ℃.

Embodiment 2

[0012] Example 2, taking the preparation of 10L differentiation medium as an example, weigh 10L of MS, 10mg of BA, 2mg of NAA, 5000mg of L-proline, 5000mg of L-glutamine, 2000mg of hydrolyzed casein, 30000mg of sucrose, 55000mg of agar powder, first add agar powder to a pot with 8L of distilled water and boil, then add MS, L-proline, L-glutamine, hydrolyzed casein, BA, NAA to the pot, wait for boiling Then add sucrose, dissolve the sucrose and make it constant to 10L, adjust the pH value to 5.8, then put the culture medium after the constant volume into the container for autoclaving, the sterilization temperature is 126°C, the pressure is 0.14kPa, Finally cooled to 1 °C.

[0013] According to the test, in the culture of mouse bougainvillea tissue, the culture period of the differentiation medium prepared by the above method is 15-25 days, which greatly shortens the culture period and increases the cell differentiation by 5-8 times.

Embodiment 3

[0014] Example 3, taking the preparation of 1L rooting medium as an example: weigh 1L of MS, 0.2mg of NAA, 500mg of L-proline, 500mg of L-glutamine, 200mg of hydrolyzed casein, and 4000mg of sucrose , 550mg of agar powder, first add agar powder to a pot with 0.8L distilled water and boil, then add MS, L-proline, L-glutamine, hydrolyzed casein, NAA to the pot, add sucrose after boiling , after the sucrose melted, the volume was fixed to 1L, and the pH value was adjusted to 5.8. Then, the culture medium after constant volume was put into the container for autoclaving. The sterilization temperature was 121°C, the pressure was 0.1kPa, and finally cooled to 1°C.

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PUM

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Abstract

The invention relates to an acanthus ilicifolius plant tissue culture medium and a preparation method thereof. A differential medium is formed by dissolving BA (Butyl Acrylate), NAA (N Aceytl Aspartate), L-proline, L-glutamine, casein hydrolysate, cane sugar and agar powder in MS. A root medium is formed by dissolving NAA, L-proline, L-glutamine, casein hydrolysate, cane sugar and agar powder in the MS. The acanthus ilicifolius plant tissue culture medium provided by the invention has the advantages of fast breeding speed, strong growth vigor of plants, good stress resistance and the like.

Description

technical field [0001] The invention relates to a plant culture medium and a preparation method. Background technique [0002] At present, the mouse bougainvillea has been widely cultivated as a mangrove afforestation plant. In actual production, the method of seed propagation is generally adopted for the rat bougainvillea. Although the seed propagation technology is widely used, there are still many shortcomings, such as time-consuming and labor-intensive, and the seedling rate is not high, which makes large-scale production difficult and cannot meet the needs of the market. Traditional seed propagation methods cannot meet the needs of large-scale production and market demand. Contents of the invention [0003] The object of the present invention is to provide a plant tissue culture medium of Bougainvillea japonica which not only has fast propagation speed, strong plant growth, low cost and is suitable for large-scale production, and its preparation method. [0004] The...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 江晨安淼徐凌丽王庆彪钟扬
Owner FUDAN UNIV
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