Noninvasive detection kit for Hashimoto's thyroiditis genes

A technology for Hashimoto's thyroiditis and a kit, applied in the field of molecular biology, can solve problems such as reduced transcriptional activity
CN103374622AInactive Publication Date: 2013-10-30解码(上海)生物医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
解码(上海)生物医药科技有限公司
Publication Date
2013-10-30
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention provides a noninvasive detection kit for detecting Hashimoto's thyroiditis genes. The kit comprises specific primers and DNA (deoxyribonucleic acid) sequencing primers for detecting genotypes of 2 mononucleotide polymorphism sites (A49G on CTLA4 gene and C-509T on IL-4 gene), a PCR (polymerase chain reaction) reaction assembly, a PCR product purification assembly, a DNA sequencing reaction assembly and the like. The kit provided by the invention detects genotypes of 2 mononucleotide polymorphism sites closely related to generation of Hashimoto's thyroiditis to evaluate the attack risk level of Hashimoto's thyroiditis, thereby preventing the generation of Hashimoto's thyroiditis in advance. The sampling method is an oral mucosa cell sampling method which is painless and noninvasive and avoids cross infection. The method has the advantage of accurate and reliable sequencing detection result, does not need to buy expensive imported special instruments, and thus, is easy to popularize.
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Description

technical field

[0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, and specifically relates to a gene detection kit for Hashimoto's thyroiditis, which evaluates the risk level of Hashimoto's thyroiditis from the genetic level according to the detection results, and guides Hashimoto's thyroiditis Early prevention and treatment of high-risk groups. Background technique

[0002] Hashimoto's thyroiditis (HT) is a clinically common autoimmune thyroiditis, manifested as a chronic inflammatory response with autoantibodies in serum and lymphocyte infiltration in the thyroid, and is a type of helper T lymphocyte (Th1 ) mediated autoimmune diseases. Both HT and Graves' disease (GD) are common autoimmune thyroid diseases (AITD). At present, it is believed that the pathogenesis of AITD is caused by the autoimmune response generated by the interaction of various environmental and genetic factors.

[0003] Cytotoxic T lymphocyte asso...

Claims

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