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Method for detecting paeoniflorin

A technology for paeoniflorin and content, which is applied in the fields of medicine and chemistry, can solve the problems of long analysis time, complex pretreatment, online monitoring of difficult paeoniflorin, etc., and achieves the effects of rapid analysis, reduced instrument noise, and accurate and reliable results.

Inactive Publication Date: 2013-10-30
TIANJIN TASLY ZHIJIAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the content determination of paeoniflorin is mainly performed by high-performance liquid chromatography (HPLC method), which has the characteristics of accurate quantification and good reproducibility, but generally requires complex pretreatment, long analysis time, and it is difficult to detect paeoniflorin in the production process. Effective online monitoring of the content of

Method used

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  • Method for detecting paeoniflorin
  • Method for detecting paeoniflorin
  • Method for detecting paeoniflorin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] 1 Instruments and reagents

[0072] BRUKER TENSOR37 Fourier transform near-infrared spectrometer (Bruker, Germany), equipped with quantitative analysis software OPUS 6.5 (Bruker, Germany); Agilent1100 high performance liquid chromatography (Agilent Technology, USA); Mettler XS105 electronic balance (Shanghai Mei Teller Toledo Instrument Co., Ltd.); Paeoniflorin standard substance (National Institute for the Control of Pharmaceutical and Biological Products, batch number: 110736-201035, in 96.5%); Water for extraction is purified water, and water for liquid phase is ultrapure water (Millipore), Chromatographic acetonitrile (Merck, Germany), and other reagents were of analytical grade.

[0073] The three batches of Radix Paeoniae Rubra were all produced in Inner Mongolia.

[0074] 2HPLC Determination of Paeoniflorin Content

[0075] 2.1 Preparation of paeoniflorin standard substance

[0076] Paeoniflorin 2.66mg (according to 96.5%) was dissolved in a 5ml volumetric fla...

Embodiment 2

[0093] 1 Instruments and reagents

[0094] Three batches of Paeoniae Alba were all produced in Anhui.

[0095] All the other are with embodiment 1.

[0096] 2HPLC Determination of Paeoniflorin Content

[0097] 2.1 Preparation of paeoniflorin standard substance

[0098] With embodiment 1.

[0099] 2.2 Preparation of calibration set samples and validation set samples

[0100] Three batches of Radix Paeoniae Alba, each batch weighed 400g of medicinal materials, added 10 times the amount of purified water into a 5L round bottom flask, decocted with a 5L electric heating mantle until it boiled slightly for one hour; after the first decoction, add 8 times Measure purified water, decoct to a slight boil for one hour. Take 10mL extract every 10min for each decoction, and the mixture of the first decoction and the second decoction is also used as a sampling point. A total of 72 samples were obtained through three batch extraction processes. Among them, 56 extracts were selected ...

Embodiment 3

[0115] 1 Instruments and reagents

[0116] The three batches of peony bark are all produced in Anhui.

[0117] All the other are with embodiment 1.

[0118] 2HPLC Determination of Paeoniflorin Content

[0119] 2.1 Preparation of paeoniflorin standard substance

[0120] With embodiment 1.

[0121] 2.2 Preparation of calibration set samples and validation set samples

[0122] Three batches of peony cortex, weigh 400g of medicinal materials for each batch, add 10 times the amount of purified water into a 5L round bottom flask, decoct with a 5L electric heating mantle until it boils slightly for one hour; after the first decoction, add 8 times the amount of purified water , and cook until slightly boiled for one hour. Take 10mL extract every 10min for each decoction, and the mixture of the first decoction and the second decoction is also used as a sampling point. A total of 74 samples were obtained through three batch extraction processes. Among them, 58 extracts were selec...

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Abstract

The invention provides a near infrared transmittance spectroscopy method for detecting paeoniflorin. The method comprises the following steps of: measuring paeoniflorin content of a calibration set sample through a high performance liquid chromatography (HPLC) method; acquiring characteristic near infrared spectrum data of the calibration set sample; establishing a related calibration model between the characteristic near infrared spectrum data of the calibration set sample and the paeoniflorin content; and finally, validating the calibration model by using a validation set sample, and measuring the paeoniflorin content of a sample to be detected by employing the calibration model.

Description

technical field [0001] The invention belongs to the fields of medicine and chemistry, and in particular relates to a near-infrared spectrum detection method and application of paeoniflorin. Background technique [0002] Near infrared (near infrared, NIR) spectroscopic analysis is a rapid detection method developed rapidly in recent years. It can directly measure the content of multiple components at the same time without complex pretreatment of the sample, which is fast, convenient, and pollution-free. , non-destructive and other advantages, has been applied in process analysis and quality control in many industrial fields. [0003] Paeoniflorin is an index component of various Chinese medicinal materials or Chinese patent medicines. At present, the content determination of paeoniflorin is mainly performed by high-performance liquid chromatography (HPLC method), which has the characteristics of accurate quantification and good reproducibility, but generally requires complex...

Claims

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Application Information

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IPC IPC(8): G01N21/35G01N30/88G01N1/44G01N30/12
Inventor 叶正良许崇瑶李德坤周大铮
Owner TIANJIN TASLY ZHIJIAO PHARMA
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