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Method for Determination of Ammonium Nitrogen in Fecal Samples

A technology of ammonium nitrogen and samples, which is applied in the field of ammonium nitrogen detection, and achieves the effects of low requirements, good reproducibility and fast determination speed

Active Publication Date: 2016-04-13
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The distillation titration method needs to adjust the distillation temperature at all times to prevent the formation of a large amount of foam or violent boiling, and finally determine the titration end point by observing the color change. The titration end point determined by different operators may be different, resulting in large errors

Method used

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  • Method for Determination of Ammonium Nitrogen in Fecal Samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment relates to a method for determining ammonium nitrogen in a stool sample, the method comprising the following steps:

[0030] Step 1. After collecting the samples, they need to be stored in a refrigerator at 4°C for testing, but the storage time should not exceed 7 days. If the test cannot be guaranteed within 7 days, it should be stored in a -18°C freezer; when measurement is required, the frozen sample should be thawed in a 4°C refrigerator and tested as soon as possible to prevent changes in volatile components and microbial decomposition.

[0031] To measure the mass percentage of dry matter: take a weighing glass plate, wash it twice with tap water and once with distilled water, dry it at 105°C to constant weight, and cool it to room temperature in a desiccator. Take the refrigerated sample at -18°C to thaw, take a numbered glass plate, weigh the plate with a weight of 16.6805g, and take the sample with a medicine spoon. The total weight of the weigh...

Embodiment 2

[0037] This embodiment relates to a method for determining ammonium nitrogen in a stool sample, the method comprising the following steps:

[0038] Step 1, collect samples and store them in a 4°C refrigerator for testing, but the storage time should not exceed 7 days. If the test cannot be guaranteed within 7 days, it should be stored in a -18°C freezer; when measurement is required, the frozen sample should be thawed in a 4°C refrigerator and tested as soon as possible to prevent changes in volatile components and microbial decomposition.

[0039] To measure the mass percentage of dry matter: take a weighing glass plate, rinse it twice with tap water and once with distilled water, dry it at 90°C to constant weight, and cool it to room temperature in a desiccator. Take the refrigerated sample at -18°C and thaw it at room temperature, take a numbered glass plate, weigh the plate with a weight of 19.1725g, and take the sample with a medicine spoon. The total weight of the weighe...

Embodiment 3

[0045] This embodiment relates to a method for determining ammonium nitrogen in a stool sample, the method comprising the following steps:

[0046] Step 1, collect samples and store them in a 4°C refrigerator for testing, but the storage time should not exceed 7 days. If the test cannot be guaranteed within 7 days, it should be stored in a -18°C freezer; when measurement is required, the frozen sample should be thawed in a 4°C refrigerator and tested as soon as possible to prevent changes in volatile components and microbial decomposition.

[0047] To measure the mass percentage of dry matter: take a weighing glass plate, rinse it twice with tap water and once with distilled water, dry it at 120°C to constant weight, and cool it to room temperature in a desiccator. Take the refrigerated sample at -18°C and thaw it at room temperature, take a numbered glass plate, weigh the plate with a weight of 16.4186g, take the sample with a medicine spoon, and the total weight of the weigh...

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Abstract

The invention discloses a method for measuring ammonium nitrogen in a faece sample. The method comprises the following steps of: step 1, collecting a sample, refrigerating, and measuring the mass percent of dry substances; step 2, taking a diluted distilled water sample, taking the supernatant, diluting, adding a saturated sodium hydroxide solution to adjust the pH value, and measuring the potential value of the diluted liquid; step 3, substituting the mass percent of the dry substances and the potential value of the diluted liquid into a standard curvilinear equation. According to the method, a water extraction liquid of a fresh faece sample is measured by using an ammonium ion sensitive type electrode, the potential value is measured after appropriate dilution, and subsequently the ionic concentration and the potential value are substituted into a calibration curve so as to confirm the ammonium ion content. The whole measuring method does not need to use expensive experimental instruments, and the experiment is simple to operate, rapid in measurement speed and applicable to popularization and use. The method has a low requirement on measurement instruments, and is simple and convenient to operate, stable in result, good in reproducibility, rapid in measurement speed, high in accuracy and applicable to popularization and use.

Description

technical field [0001] The invention belongs to the technical field of ammonium nitrogen detection methods, and in particular relates to a method for measuring ammonium nitrogen in feces samples. Background technique [0002] Ammonium nitrogen content in manure samples refers to the ammonium nitrogen content in poultry manure that can be directly used by plants. Ammonium nitrogen is a kind of inorganic nitrogen, which is easily soluble in water, has fast fertilizer effect, can be directly absorbed by crops and is not easily lost in the soil. With the expansion of poultry breeding scale, a large amount of poultry manure will inevitably be produced, which will bring serious harm to the environment if not handled properly. However, if it is effectively treated and utilized as a resource, it can also turn waste into treasure. As one of the basic physiological and biochemical indicators, the content of ammonium nitrogen has practical significance for its research. [0003] At ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/00
Inventor 刘荣厚蔡文飞王璐
Owner SHANGHAI JIAOTONG UNIV