Method for preparing high-viscosity chitosan quick hemostatic patch

A chitosan, high-viscosity technology, applied in medical science, bandages, absorbent pads, etc., can solve the problems of limitation, reduced mechanical strength, slow hemostasis, etc., to achieve the effect of strengthening hemostasis and mechanical strength, and controlling the amount of bleeding

Inactive Publication Date: 2013-11-13
黄耀江 +2
5 Cites 7 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0008] The preparation of this kind of chitosan hemostatic material mainly enhances plasticity and swelling by adding plasticizers and chemical modification of some chitosan, but for wound...
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Abstract

The invention discloses a method for preparing a high-viscosity chitosan quick hemostatic patch. The method comprises the following steps: preparing chitosan gel from high-viscosity chitosan having a deacetylation degree in the range from 80% to 90%, drying the chitosan gel and then immersing one single side of the chitosan gel into a mixed solution of chloroacetic acid and isopropanol for carboxymethylation, and then soaking the chitosan gel into a 30% glycerol solution for 30 minutes and then draining; and finally, carrying out a cross-linking reaction by using a calcium chloride solution so as to introduce calcium ions and thrombin, and then carrying out vacuum freeze-drying, thereby obtaining the high-viscosity chitosan quick hemostatic patch. Raw materials used for the process of the method are low in cost; the process is simple and convenient, and short in period; the obtained chitosan hemostatic patch is high in adhesiveness, and capable of quickly stopping bleeding of a wound under the promotion effect of the calcium ions and the thrombin.

Application Domain

Technology Topic

Examples

  • Experimental program(2)

Example Embodiment

[0021] Example 1:
[0022] Take 4g of chitosan, the degree of deacetylation is 80%, the viscosity is 2000mPa·s, and the viscosity average molecular weight is 3.8×10 6 , Add 200mL of 2% acetic acid solution, stir at room temperature (20~25℃) until completely dissolved, prepare 2% chitosan solution, let stand to degas, then filter to obtain clear chitosan solution, and bake at 70℃ Dry for 1 hour, then pour the above chitosan solution into a petri dish, and put it in the precipitation solution overnight (the precipitation solution is an absolute ethanol solution with glycerol concentration 5% and NaOH concentration 0.05%) until the chitosan is completely Precipitation to form chitosan gel, rinse with deionized water to neutral. Add chloroacetic acid and isopropanol (mass ratio 1:10) into airtight glassware, heat to 70℃ after alkalization, put the previously obtained chitosan gel in and react for 30min, take it out, and use enough ethanol Wash repeatedly many times. Put the prepared chitosan gel into a 30% mass concentration glycerol solution for plasticizing reaction for 6 hours, take it out and drain it, and dry it at 70℃ for 3 hours. Finally, immerse the chitosan gel in 0.5% The cross-linking reaction was carried out in a solution with the concentration of calcium chloride and the enzyme activity of thrombin at 4000IU/mL for 30 minutes, drained and vacuum freeze-dried to obtain a high-viscosity chitosan quick hemostatic patch.

Example Embodiment

[0023] Example 2:
[0024] Take 4g of chitosan, the degree of deacetylation is 90%, the viscosity is 600mPa·s, and the viscosity average molecular weight is 3.0×10 6 , Add 200mL of 2% acetic acid solution, stir at room temperature (20~25℃) until completely dissolved, prepare 2% chitosan solution, let stand to degas, then filter to obtain clear chitosan solution, and bake at 70℃ Dry for 1 hour, then pour the above chitosan solution into a petri dish, and put it in the precipitation solution overnight (the precipitation solution is an absolute ethanol solution with glycerol concentration 5% and NaOH concentration 0.05%) until the chitosan is completely Precipitation to form chitosan gel, rinse with deionized water to neutral. Add chloroacetic acid and isopropanol (mass ratio 1:10) into airtight glassware, heat to 70℃ after alkalization, put the previously obtained chitosan gel in and react for 30min, take it out, and use enough ethanol Wash repeatedly many times. Put the prepared chitosan gel into a 30% mass concentration glycerol solution for plasticizing reaction for 6 hours, take it out and drain it, and dry it at 70℃ for 3 hours. Finally, immerse the chitosan gel in 0.5% The cross-linking reaction was carried out in a solution with a concentration of calcium chloride and thrombin enzyme activity of 5000 IU/mL for 30 minutes, drained and vacuum freeze-dried to obtain a high-viscosity chitosan quick hemostatic patch.
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PUM

PropertyMeasurementUnit
Viscosity600.0 ~ 2000.0mPa·s
tensileMPa
Particle sizePa
strength10

Description & Claims & Application Information

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the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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