Method for preparing high-viscosity chitosan quick hemostatic patch

[0021] Example 1:

[0022] Take 4g of chitosan, the degree of deacetylation is 80%, the viscosity is 2000mPa·s, and the viscosity average molecular weight is 3.8×10 6 , Add 200mL of 2% acetic acid solution, stir at room temperature (20~25℃) until completely dissolved, prepare 2% chitosan solution, let stand to degas, then filter to obtain clear chitosan solution, and bake at 70℃ Dry for 1 hour, then pour the above chitosan solution into a petri dish, and put it in the precipitation solution overnight (the precipitation solution is an absolute ethanol solution with glycerol concentration 5% and NaOH concentration 0.05%) until the chitosan is completely Precipitation to form chitosan gel, rinse with deionized water to neutral. Add chloroacetic acid and isopropanol (mass ratio 1:10) into airtight glassware, heat to 70℃ after alkalization, put the previously obtained chitosan gel in and react for 30min, take it out, and use enough ethanol Wash repeatedly many times. Put the prepared chitosan gel into a 30% mass concentration glycerol solution for plasticizing reaction for 6 hours, take it out and drain it, and dry it at 70℃ for 3 hours. Finally, immerse the chitosan gel in 0.5% The cross-linking reaction was carried out in a solution with the concentration of calcium chloride and the enzyme activity of thrombin at 4000IU/mL for 30 minutes, drained and vacuum freeze-dried to obtain a high-viscosity chitosan quick hemostatic patch.

[0023] Example 2:

[0024] Take 4g of chitosan, the degree of deacetylation is 90%, the viscosity is 600mPa·s, and the viscosity average molecular weight is 3.0×10 6 , Add 200mL of 2% acetic acid solution, stir at room temperature (20~25℃) until completely dissolved, prepare 2% chitosan solution, let stand to degas, then filter to obtain clear chitosan solution, and bake at 70℃ Dry for 1 hour, then pour the above chitosan solution into a petri dish, and put it in the precipitation solution overnight (the precipitation solution is an absolute ethanol solution with glycerol concentration 5% and NaOH concentration 0.05%) until the chitosan is completely Precipitation to form chitosan gel, rinse with deionized water to neutral. Add chloroacetic acid and isopropanol (mass ratio 1:10) into airtight glassware, heat to 70℃ after alkalization, put the previously obtained chitosan gel in and react for 30min, take it out, and use enough ethanol Wash repeatedly many times. Put the prepared chitosan gel into a 30% mass concentration glycerol solution for plasticizing reaction for 6 hours, take it out and drain it, and dry it at 70℃ for 3 hours. Finally, immerse the chitosan gel in 0.5% The cross-linking reaction was carried out in a solution with a concentration of calcium chloride and thrombin enzyme activity of 5000 IU/mL for 30 minutes, drained and vacuum freeze-dried to obtain a high-viscosity chitosan quick hemostatic patch.